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目的:初步探讨趋化因子受体(chemokine receptor)与原发性胆汁性肝硬化(PBC,primary biliary cirrhosis)发生发展的关系。方法:采用实时荧光定量PCR和流式细胞术,分别从基因转录和蛋白表达水平检测60例PBC、60例乙型肝炎肝硬化患者(疾病对照)和60例健康对照者的外周血单个核细胞(PBMCs)中CCR1、CCR3和CCR5的表达;生化常规测定PBC患者和健康对照者的总胆红素(TBIL)、γ-谷氨酰基转移酶(γ-GT)水平,分析它们与趋化因子之间的相关性。结果:PBC患者PBMCs中的CCR1、CCR3、CCR5的mRNA和蛋白表达均明显低于健康对照组和疾病对照组(P<0.05),而疾病对照组和健康对照组PBMCs中CCR1、CCR3、CCR5 mRNA和蛋白表达无统计学差异(P>0.05);PBC患者Ⅲ、Ⅳ期PBMCs中的CCR1、CCR3、CCR5的mRNA和蛋白表达较Ⅰ、Ⅱ期明显降低(P<0.05);PBC患者CCR1蛋白表达水平与TBIL水平显著负相关(r=-0.445,P<0.01),与γ-GT无线性相关关系(r=-0.230,P>0.05);CCR3蛋白表达水平与TBIL水平无线性相关关系(r=-0.173,P>0.05),与γ-GT水平负相关(r=-0.295,P<0.05);CCR5蛋白表达水平与TBIL、γ-GT水平均负相关(r=-0.531,P<0.01;r=-0.665,P<0.01)。结论:CCR1、CCR3、CCR5的表达与PBC的发生发展存在一定的相关性,可能参与了PBC的调控机制,为PBC的诊断和预防提供了新线索。
Objective: To investigate the relationship between the chemokine receptor and the development of primary biliary cirrhosis (PBC). METHODS: Peripheral blood mononuclear cells (PBMC) from 60 patients with PBC, 60 patients with hepatitis B cirrhosis (disease control) and 60 healthy controls were detected by real-time fluorescence quantitative PCR and flow cytometry respectively. (PBMCs). The levels of total bilirubin (TBIL) and γ-glutamyltransferase (γ-GT) in PBC patients and healthy controls were determined by biochemical methods. The levels of CCR1, The correlation between. Results: The mRNA and protein expressions of CCR1, CCR3 and CCR5 in PBMCs of patients with PBC were significantly lower than those in healthy controls and disease controls (P <0.05), while the expression of CCR1, CCR3 and CCR5 mRNA in PBMCs of disease control group and healthy control group (P> 0.05). The mRNA and protein expressions of CCR1, CCR3 and CCR5 in PBMCs in stage Ⅲ and Ⅳ of PBC patients were significantly lower than those in stage Ⅰ and Ⅱ (P <0.05). The expression of CCR1 protein (R = -0.445, P <0.01), but no correlation with TBIL (r = -0.230, P> 0.05). There was no linear correlation between the level of CCR3 protein and the level of TBIL (r (R = -0.295, P <0.05). The expression of CCR5 protein was negatively correlated with TBIL and γ-GT levels (r = -0.531, P <0.01) ; r = -0.665, P <0.01). Conclusion: The expressions of CCR1, CCR3 and CCR5 are related to the occurrence and development of PBC, which may be involved in the regulatory mechanism of PBC, providing new clues for the diagnosis and prevention of PBC.