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本文应用微量淋巴细胞增生试验,比较了体内给药组和对照组日本血吸虫感染鼠脾细胞在体外对促有丝分裂原和抗原刺激的反应性。两次用不同性别的小鼠分别进行的实验结果基本一致,均证明了“425”制剂体内给药能显著增强感染鼠脾细胞在体外对细菌脂多糖(LPS)刺激的反应性。体外培养中直接加入“425”制剂,低剂量的“425”制剂略能增强感染鼠脾细胞 DNA 的合成,增加剂量则呈现抑制作用;“425”制剂若与促有丝分裂原或抗原同时加入稗细胞培养物中,则抑制丝裂原或抗原的促分裂作用。实验结果提示,“425”制剂增强感染鼠 B 细胞介导的免疫应答尤其是非特异性免疫应答可能与感染鼠肝组织内虫卵肉芽肿病变的控制有关。
In this study, we measured the reactivity of mitosin and antigen-stimulated cells in spleen cells infected with Schistosoma japonicum in vivo and in the control group by using microlipoproliferation assay. The results of the two experiments with different sexes were basically the same, and it was proved that in vivo administration of “425” formulation significantly enhanced the responsiveness of infected mouse spleen cells to bacterial lipopolysaccharide (LPS) stimulation in vitro. In vitro culture, the “425” preparation was added directly. The low dosage of “425” preparation slightly enhanced the DNA synthesis in the spleen cells infected with the “425” preparations and the inhibition increased at the dose of “425” .If barley cells were added with mitogen or antigen In culture, the mitogenic or antigen-promoting mitosis is inhibited. The experimental results suggest that the “425” preparation enhances the immune response mediated by infected B cells, especially the nonspecific immune response may be related to the control of the egg granuloma lesions in the infected rat liver tissue.