论文部分内容阅读
甲胎蛋白检测在肝癌免疫诊断中的广泛应用，要求人们进一步深入了解甲胎蛋白在正常及病理情况下的发生发展规律。除了进行其血清含量测定以外，尚需了解其产生部位、产生时间、产生后的分布、不同分化程度的肝癌细胞内含量有无差异等等，这些都需要寻找能给甲胎蛋白以正确的细胞内定位的方法。一般组织学及组织化学方法难以达到这一要求。随着免疫学研究的深入，使抗元、抗体、补体在疾病中的作用更显重要，为了了解免疫因子在发病中的意义，多年来人们梦想用免疫学方法在组织中看到抗元、抗体或补体。经过了几十年的努力，产生了萤光抗体法，基本上满足了上述要求，其特异性强、灵敏度高、定位正确，而且方法逐渐简化，目前已应用于医学的各个领域。我们试用萤光抗体法来进行甲胎蛋白的组织内定位，报导如下。
AFP detection in the diagnosis of liver cancer in a wide range of applications require people to further understand the development of alpha fetoprotein in normal and pathological conditions. In addition to the determination of its serum content, but also need to understand its location, time of production, the distribution after birth, the different levels of differentiation of liver cancer cells in the content of whether there is any difference, etc., these need to find the right to give alpha-fetoprotein cells Internal positioning method. General histology and histochemical methods are difficult to meet this requirement. With the deepening of immunological research, anti-Yuan, antibodies, complement in the role of the disease is more important, in order to understand the significance of immune factors in the pathogenesis, over the years people have dreamed of immunological methods in the organization to see anti-Yuan, Antibody or complement. After decades of efforts, a fluorescent antibody method has been developed that substantially meets the above requirements. Its specificity, sensitivity, positioning accuracy and method simplification have been used in various fields of medicine. We tried fluorescent antibody method for the localization of alpha-fetoprotein tissue, reported as follows.