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以抗青枯病茄子自交系‘E-31’为材料,利用病毒诱导的基因沉默(virus induced gene silencing,VIGS)技术沉默抗病材料中调控抗病相关的信号基因,研究其在茄子抗青枯病反应中的作用。q RT-PCR结果表明,与对照植株相比,VIGS诱导基因沉默植株,目的基因的表达量均下降。MAPK级联途径相关基因MKK2和MAPK6,SA途径中的信号基因PAD4、NPR1、SGT1、TGA和Glu A,以及WRRY转录因子基因WRKY70沉默,接种青枯雷尔氏菌(Ralstonia solanacearum)后植株出现不同程度的枯萎,而对照植株无变化。MAPK级联途径相关基因MAPK3和MAPK4,SA途径中的信号基因NDR1,ET途径中的信号基因EIN2和EIL1,JA途径信号基因JAR1沉默后,植株均未出现萎蔫症状。研究结果表明,MKK2、MAPK6、PAD4、NPR1、SGT1、TGA、Glu A和WRKY70等基因在茄子调控抗青枯病反应中起正调控作用,而MAPK3、MAPK4、NDR1、EIL1、EIN2和JAR1等基因可能未参与或起负调作用,推断茄子‘E-31’调控青枯病信号途径可能主要依赖于SA途径。
The anti-bacterial wilt eggplant inbred line ’E-31’ was used as a material, and the virus-induced gene silencing (VIGS) technique was used to silence the signal genes related to disease resistance in disease- The role of bacterial wilt response. q RT-PCR results showed that, compared with the control plants, VIGS-induced gene silencing plants, the expression of the target gene were decreased. The signal genes PAD4, NPR1, SGT1, TGA and Glu A in MKK2 and MAPK6, SA pathway of MAPK cascade pathway and the WRRY transcription factor gene WRKY70 were silenced, and the plants appeared different after inoculation with Ralstonia solanacearum Withered, while the control plants did not change. MAPK3 and MAPK4 in MAPK cascade pathway, signal pathway genes in NDR1 and ET pathway, EIN2 and EIL1 in ET pathway, and JAR1 signaling pathway in JA1 pathway, all the plants showed no symptoms of wilting. The results showed that genes such as MKK2, MAPK6, PAD4, NPR1, SGT1, TGA, Glu A and WRKY70 play a positive role in the regulation of eggplant resistance to bacterial wilt. However, genes such as MAPK3, MAPK4, NDR1, EIL1, EIN2 and JAR1 May not participate or play a negative role, concluded eggplant ’E-31’ regulation of bacterial wilt signal pathway may depend on the SA pathway.