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Objective: To report the birth of a healthy baby after transfer of blastocysts derived from frozen eggs and frozen spermatozoa. Design: Case report. Setting: University-based assisted reproduction center. Patient(s): A 37-year-old woma n with secondary infertility of 4 years’duration. Intervention(s): Retrieved oo cytes were cryopreserved in 1.5 M 1,2-propanediol (PROH) and 0.3 M sucrose by a slow freezing-rapid thawing protocol, semen cryopreservation, and insemination by intracytoplasmic sperm injection (ICSI) at 4 hours after thawing. Main Outco me Measure(s): Fertilization and embryo development to blastocyst stage, pregnan cy, and outcome. Result(s): Ten of 14 frozen oocytes survived after thawing. Eig ht of them were fertilized by performing ICSI and three developed into 7-to 8- cell embryos on day 3. Two of these embryos developed into blastocysts on day 5 and were transferred. This resulted in a successful pregnancy and the delivery o f a healthy baby boy. Conclusion(s): This case demonstrates the feasibility of i nseminating the frozen-thawed human oocytes after 4 hours of in vitro culture a nd the zygotes derived from frozen oocytes and frozen spermatozoa can be culture d to blastocysts resulting in the live birth of a healthy baby boy.
Objective: To report the birth of a healthy baby after transfer of blastocysts derived from frozen eggs and frozen spermatozoa. Design: Case report. Setting: University-based assisted reproduction center. Patient (s): A 37-year-old woma n with Secondary infertility of 4 years’ duration. Intervention (s): Retrieved o cytes were cryopreserved in 1.5 M 1,2-propanediol (PROH) and 0.3 M sucrose by a slow freezing-rapid thawing protocol, semen cryopreservation, and insemination by intracytoplasmic sperm Results (s): Ten of 14 frozen oocytes survived after thawing. Eig ht of them (ICSI) at 4 hours after thawing. Main Outco me Measure (s): Fertilization and embryo development to blastocyst stage, pregnan cy, and outcome. were fertilized by performing ICSI and three developed into 7-to 8-cell embryos on day 3. Two of these embryos developed into blastocysts on day 5 and were transferred. This resulted in a successful pregnancy and the delivery of a healthy baby boy. Conclusion ( s): This c ase demonstrates the feasibility of i nseminating the frozen-thawed human oocytes after 4 hours in vitro culture a nd the zygotes derived from frozen oocytes and frozen spermatozoa can be culture d to blastocysts resulting in the live birth of a healthy baby boy.