应用斑点杂交法从甲型肝炎及其复发患者的粪便中检测HAVRNA

• 作 者: 窦晓光 吴海滨 李智伟 王兆荃
• 机 构: 中国医科大学第二临床学院传染科
• 出 处: 辽宁医学杂志
• 发表日期: 1998年01期
• 关键词: 斑点杂交 HAVRNA 血清转氨酶 甲肝病毒 致病因子 自限性疾病 急性期 病毒感染 杂交法 慢性化

本文应用３２Ｐ标记的ＨＡＶｃＤＡＮ探针进行斑点杂交，检测５０例急性甲型肝炎及４例复发患者粪便中的ＨＡＶＲＮＡ，同时检测抗ＨＡＶＩｇＭ、血清转氨酶及胆红素。结果发现，４例复发的甲型肝炎患者复发时间为病愈后３０～５０天，此时血清转氨酶及胆红素均再次升高，抗ＨＡＶＩｇＭ阳性，复发后第１周的粪便中可再次检出ＨＡＶＲＮＡ。提示ＨＡＶ可能为甲型肝炎临床复发的致病因子。

In this paper, HAVcDAN probes labeled with 32P were used for dot blot hybridization. HAV RNAs in 50 cases of acute hepatitis A and 4 cases of recurrent patients were detected. Anti-HAVIgM, serum transaminases and bilirubin were also detected. The results showed that the recurrence time of 4 recurrent hepatitis A patients was 30 to 50 days after recovery, at which time the serum aminotransferases and bilirubin were raised again, anti-HAVIgM positive, again in the first week after recurrent stool HAVRNA out. Suggesting that HAV may be the causative agent of hepatitis A clinical relapse.