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目的:研究糖尿病大鼠心肌线粒体氧自由基异常的机制。方法:测定正常对照组和链脲佐菌素(STZ)诱导的2型糖尿病大鼠心肌线粒体中一氧化氮(NO)、丙二醛(MDA)、细胞色素C(Cyt-C)和线粒体钙含量及超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)活性,并用透射电镜观察病理学改变。结果:模型组心肌线粒体NO、NOS、MDA、钙和胞浆细胞色素C均显著高于对照组(P<0.05);线粒体细胞色素C和SOD均显著低于对照组(P<0.05)。电镜下,模型组肌原纤维排列紊乱,部分融解消失,线粒体肿胀变性,数目增多。结论:氧自由基异常及脂质过氧化作用参与糖尿病心肌的线粒体损伤;Cyt-C和线粒体钙的改变也是糖尿病心肌线粒体损伤的因素。
Objective: To investigate the mechanism of mitochondrial oxygen free radical in diabetic rats. Methods: The level of myocardial nitric oxide (NO), malondialdehyde (MDA), cytochrome C (Cyt-C) and mitochondrial calcium in myocardium of type 2 diabetic rats induced by streptozotocin (STZ) Content and the activity of superoxide dismutase (SOD) and nitric oxide synthase (NOS). The pathological changes were observed by transmission electron microscope. Results: The levels of NO, NOS, MDA, calcium and cytoplasmic cytochrome C in myocardial mitochondria in model group were significantly higher than those in control group (P <0.05). The mitochondrial cytochrome C and SOD were significantly lower than those in control group (P <0.05). Electron microscope, the model group of myofibrils arranged in disorder, part of the melt disappear, mitochondrial swelling degeneration, the number increased. Conclusion: Oxygen free radical abnormalities and lipid peroxidation are involved in mitochondrial damage of diabetic myocardium. The changes of Cyt-C and mitochondrial calcium are also the factors of mitochondrial damage in diabetic myocardium.