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目的:研究在模拟人体生理条件下5-羟甲基糠醛(5-HMF)和牛血清白蛋白(BSA)结合反应的特征。方法:采用荧光光谱法和紫外光谱法。取一定浓度的BSA与不同浓度的5-HMF溶液反应(27、37℃下),测定荧光强度和吸光度,再以此通过S理te,r测n-V定ol了m其er公相式互计结算合结时合5-常HM数F和与结B合SA位作点用数距,通离过。计结算果热:2力7、学37数℃据下探5讨-H5M-HFM与FB与SAB的SA结的合相常互数作K用分机别制为;运1.4用×1F0?3rsLt.erm能ol量-1转和移7.9原×103L.mol-1,结合位点数分别为0.61和0.78;根据基本热力学参数判断二者主要通过典型的疏水作用力发生相互作用,作用距离为5.6nm。结论:5-HMF对BSA有较强的荧光猝灭作用,该过程为静态猝灭,二者在试验浓度范围内形成了复合物,从而使5-HMF可以BSA为载体进行贮存和运输。
OBJECTIVE: To study the characteristics of 5-HMF and bovine serum albumin (BSA) binding reactions under simulated human physiology. Methods: Fluorescence and UV spectroscopy were used. Take a certain concentration of BSA with different concentrations of 5-HMF solution reaction (27,37 ° C), the fluorescence intensity and absorbance were measured, and then through the S te, r measured nV set olm er er public phase Settlement when combined with 5 - constant HM F and the junction B with the SA bit for a few with points, pass away. Calculated results heat: 2 force 7, learn 37 ℃ under the probe 5 discussion-H5M-HFM and FB and SAB the SA junction of the phase combination of each other for K with the extension system; transport 1.4 × 1F0? 3rsLt .erm was able to move from ol to -1 and shift to 7.9 × 103L.mol-1, respectively. The number of binding sites was 0.61 and 0.78, respectively. According to the basic thermodynamic parameters, the interaction between the two mainly through the typical hydrophobic interaction force was 5.6 nm. CONCLUSION: 5-HMF has strong fluorescence quenching effect on BSA. The process is quiescent quenching. Both complexes form complexes within the experimental concentration range, so that 5-HMF can be stored and transported by BSA as a carrier.