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目的探讨甲胎蛋白(AFP)-mRNA在原发性肝癌(HCC)患者外周血中的表达和临床意义。方法采用荧光定量逆转录聚合酶链反应(RT-PCR)方法,检测25例HCC患者外周血中AFP-mRNA的表达。结果HCC患者外周血中AFP-mR-NA含量显著高于肝炎和肝硬化病人(P<0.01)。HCC患者外周血中AFP-mRNA含量与病人的肝外转移明显相关(P<0.05)。HCC患者外周血中AFP-mRNA含量与病人的临床分期肿瘤直径、数目、血清AFP水平无明显相关(P>0.05)。结论AFP-mRNA可作为血循环中有肝癌细胞或肝癌细胞的标志物,对HCC患者,阳性预示有血源性转移的可能,可作为预测肝癌复发、转移的参考指标。
Objective To investigate the expression and clinical significance of AFP-mRNA in peripheral blood of patients with primary hepatocellular carcinoma (HCC). Methods The expression of AFP-mRNA in peripheral blood of 25 patients with HCC was detected by fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results The level of AFP-mR-NA in peripheral blood of patients with HCC was significantly higher than that of patients with hepatitis and cirrhosis (P <0.01). The level of AFP-mRNA in peripheral blood of HCC patients was significantly correlated with extrahepatic metastasis (P <0.05). The level of AFP-mRNA in peripheral blood of patients with HCC was not significantly correlated with the clinical stage and tumor diameter, number of patients and serum AFP level (P> 0.05). Conclusion AFP-mRNA can be used as a marker of hepatocellular carcinoma cells or hepatocellular carcinoma cells in the blood circulation. Positive staining of AFP-mRNA may indicate the possibility of blood-borne metastasis in HCC patients, which may serve as a reference index for predicting the recurrence and metastasis of HCC.