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目的 研究血清庚型肝炎病毒核糖核酸 (HGVRNA)阳性或阴性肝病肝组织中HGV抗原 (HGVAg)的表达状况及其临床意义。 方法 应用抗HGV非结构 5区 (NS5)单克隆抗体(McAb) ,以免疫组织化学BA法检测肝细胞中HGVNS5抗原。结果 HGVAg阳性染色为棕黄色 ,呈细小颗粒状位于肝细胞胞浆 ,肝组织中阳性肝细胞呈散在分布 ;在血清HGVRNA阳性和阴性肝组织中HGVAg的检出率分别为 6 3 6 4% (7/ 11例 )和 5 6 2 % (5 / 89例 )。结论 HGV属于一种嗜肝病毒 ,HGVAg在肝组织的表达与血清HGVRNA关系密切。
Objective To study the expression and clinical significance of HGV antigen (HGV) in hepatic tissue of patients with positive or negative hepatocellular hepatitis virus (HGVRNA). Methods Anti-HGV monoclonal antibody against non-structural 5 (NS5) was used to detect HGVNS5 antigen in hepatocytes by immunohistochemistry. Results The HGVAg positive staining was brown and yellow, with small granularity located in the cytoplasm of hepatocytes. The positive hepatocytes in the liver tissue were scattered. The positive rates of HGV Ig in positive and negative HGV RNA were 63.64% 7/11 cases) and 56.2% (5/89 cases). Conclusion HGV belongs to a kind of hepadnavirus. The expression of HGVAg in liver tissue is closely related to serum HGVRNA.