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采用SDS—聚丙烯酰胺凝胶电泳法和半微量凯氏定氮法,对在形态学特征和穗部产量构成因子已稳定的小麦胚培无性系IE_5代醇溶蛋白、谷蛋白亚基和蛋白质含量进行测定和分析的研究。结果表明,小麦籽粒胚乳醇溶蛋白和谷蛋白电泳谱带发生较大变化,出现三种变异类型:1)、增加新谱带;2)、亲本原有特异性谱带缺失;3)、亲本原有特异性谱带强度改变,高分子量和低分子量蛋白亚基发生变化。籽粒蛋白质含量出现范围较广(9.75％～16.20％)的变异,有7.69％的株系蛋白质含量明显超过对照,并与早熟大粒结合。表明胚培无性系变异的诱导和筛选,可以作为小麦品种品质改良的一种手段。
SDS-polyacrylamide gel electrophoresis and semi-micro Kjeldahl method were used to study the effects of morphological traits and spike yield on the stability of wheat germ clones IE_5 gliadin, glutenin subunits and proteins Content determination and analysis of the study. The results showed that there were three major changes in the wheat gliadin and gluten electrophoresis bands, including three types of variation: 1) the addition of a new band; 2) the loss of the original specific band of the parent; 3) Original intensity of the specific band changes, high molecular weight and low molecular weight protein subunits change. The variation of protein content in grains ranged from 9.75% to 16.20%. 7.69% of the total protein content in the lines was significantly higher than that in the control and was associated with the premature grains. Induction of embryo culture clonal variation and selection, can be used as a means of improving the quality of wheat varieties.