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目的研究90 d摄入炔苯酰草胺原药对大鼠的亚慢性毒性作用,确定其未观察到损害作用剂量,为安全生产及慢性毒性实验提供剂量参考。方法将80只SD大鼠(雌雄各半)按体重随机分为4组,分别为炔苯酰草胺原药31.25、125.00和500.0 mg/kg·bw·d和正常对照组。在实验结束后处死实验大鼠,同时检测体重、血常规、血清生化和脏器系数等指标,并对结果进行统计学处理。结果中、高剂量组雄性大鼠和高剂量组雌性大鼠周平均体重从第1周开始、中剂量组雌性大鼠周平均体重从第2周开始至实验结束均明显低于对照组;中、高剂量组雄性大鼠WBC、HGB、PLT、淋巴细胞及高剂量组RBC明显低于对照组,中、高剂量组中性细胞明显高于对照组,且存在剂量-反应关系,高剂量雌性大鼠的RBC、HGB、PLT明显低于对照组;雄性大鼠高剂量组的ALP、CHOL、CHE明显高于对照组,中、高剂量组的CREA、TG明显低于对照组,雌性大鼠高剂量组的ALP明显高于对照组,低、中、高剂量组的CHE明显高于对照组,存在剂量-反应关系;雄性大鼠中、高剂量组的脑、肝、肺、肾、肾上腺、睾丸等脏器系数明显高于对照组,雌性大鼠高剂量组的脑及中、高剂量组的肝肾等脏器系数明显高于对照组(P<0.05,P<0.01)。结论炔苯酰草胺原药对雄、雌性大鼠经口亚慢性染毒剂量为125.00 mg/kg·bw及以上时,对大鼠有毒性效应;90 d喂养炔苯酰草胺草胺原药对大鼠未观察到损害作用剂量为31.2 5mg/kg·bw。
Objective To study the subchronic toxic effects of propyzamide 90 d on rats, to determine the dose of no damage observed, and to provide dose reference for safe production and chronic toxicity test. Methods Eighty male Sprague-Dawley rats were randomly divided into 4 groups according to body weight: 31.25, 125.00 and 500.0 mg / kg · bw · d of propyzamide and normal control group respectively. At the end of the experiment, the experimental rats were sacrificed and the body weight, blood routine, serum biochemistry and organ coefficient were measured, and the results were statistically analyzed. Results The mean weekly body weight of female rats in high dose group and high dose group began from the first week. The average weekly body weight of the female rats in the middle dose group was significantly lower than that of the control group from the second week to the end of the experiment. , High dose group of male rats WBC, HGB, PLT, lymphocytes and high dose group RBC was significantly lower than the control group, medium and high dose group of neutrophils was significantly higher than the control group, and there is dose-response relationship, high-dose female The RBC, HGB and PLT in rats were significantly lower than those in control group. The levels of ALP, CHOL and CHE in high dose group were significantly higher than those in control group. The CREA and TG levels in middle and high dose group were significantly lower than those in control group The ALP in the high dose group was significantly higher than that in the control group, while the CHE in the low, medium and high dose groups was significantly higher than that in the control group, with dose-response relationship. The levels of CHE in brain, liver, lung, kidney, adrenal , Testis and other organ coefficients were significantly higher than the control group, high-dose group of female rats brain and medium and high dose group of liver and kidney and other organ coefficient was significantly higher than the control group (P <0.05, P <0.01). Conclusion The propynthamide drug has a toxic effect on rats when administered subcutaneously in a dose of 125.00 mg / kg · bw or above for both male and female rats. The propynidin The medicine did not observe the damaging effect dose to the rat is 31.2 5mg / kg · bw.