论文部分内容阅读
试验比较不同来源白术提取物对水杨酸、间羟基苯甲酸、对羟基苯甲酸、对香豆酸、阿魏酸、丁香酸、肉桂酸、芥子酸和绿原酸9种酚酸物质的高效液相色谱法检测及白术来源对其酚酸物质含量的影响。试验采用ACQUITY UPLC?BEH C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相A相为0.3%乙酸水溶液、流动相B相为90%甲醇水溶液,梯度洗脱,流速为1.0 m L/min,DAD检测波长为285 nm,柱温为40℃。检测方法显示:9种酚酸物质的检测线性范围为0.02~500 mg/L;检出限(S/N=3)在0.002~0.01 mg/kg之间;在加标浓度为10~80 mg/kg条件下,加标回收率(N=9)在77.78%~100.47%之间,检测方法灵敏有效;标准品进样6次的结果RSD均小于5%,表明精密度良好。检测结果表明,不同来源白术提取物得到的白术提取物中水杨酸、间羟基苯甲酸、对羟基苯甲酸、对香豆酸、阿魏酸、丁香酸、肉桂酸、芥子酸和绿原酸9种白术提取物酚酸物质的总含量以浙江新昌产白术提取物最高。
Experiments comparing different sources of Atractylodes extract of salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, p-coumaric acid, ferulic acid, syringic acid, cinnamic acid, erucic acid and chlorogenic acid 9 kinds of phenolic acids efficient The Influence of Liquid Chromatography and Atractylodes Source on the Content of Phenolic Acids. The ACQUITY UPLC BEH C 18 column (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase A was 0.3% acetic acid aqueous solution and the mobile phase B was 90% aqueous methanol. The mobile phase was gradient elution at a flow rate of 1.0 m L / min, DAD detection wavelength of 285 nm, column temperature of 40 ℃. The detection methods showed that the linear range of the nine phenolic acids was 0.02-500 mg / L, the detection limit (S / N = 3) was between 0.002-0.01 mg / kg, the spiked concentration was 10-80 mg / kg, the spiked recoveries (N = 9) ranged from 77.78% to 100.47%. The detection methods were sensitive and effective. The RSDs of the standard samples were all less than 5% after 6 injections, indicating good precision. The results show that the different sources of Atractylodes extract Atractylodes salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, p-coumaric acid, ferulic acid, syringic acid, cinnamic acid, erucic acid and chlorogenic acid The total content of phenolic acids in nine kinds of Atractylodes macrocephalae extract was the highest in Atractylodes macrocephalae from Xinchang, Zhejiang Province.