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目的:探讨头孢曲松通过抑炎通路发挥神经保护作用的机制。方法:45只雄性SD大鼠,随机分为3组(n=15),预处理组在大鼠大脑中动脉阻断(MCAO)前5 d每日给予腹腔注射头孢曲松缓冲液(200 mg/kg),观察局灶性脑缺血2 h再灌注24 h后,各组间大鼠神经行为学评分、脑梗死容积变化、小胶质细胞和IL-1β的数量变化。结果:再灌注24 h后,头孢曲松预处理组可改善大鼠神经行为学评分和脑梗死面积(P<0.05),小胶质细胞活化数量减少(P<0.05),ELISA检测IL-1β分泌数量有显著下降趋势,但无统计学差异(P=0.18)。结论:头孢曲松可以部分抑制小胶质细胞活化,减少IL-1β释放发挥神经保护作用。
Objective: To investigate the mechanism of ceftriaxone exerting neuroprotective effect through anti-inflammatory pathway. Methods: Forty five male Sprague Dawley rats were randomly divided into 3 groups (n = 15). The rats in the preconditioning group were given intraperitoneal injection of ceftriaxone buffer (200 mg / kg). The neurobehavioral scores, volume changes of cerebral infarction and the changes of microglial cells and IL-1β were observed after focal cerebral ischemia 2 h and reperfusion 24 h. Results: After 24 h of reperfusion, the ceftriaxone preconditioning group could improve neurobehavioral score and infarct size (P <0.05), decrease the number of microglia activation (P <0.05), and detect IL-1β The number of secretions showed a significant downward trend, but no statistical difference (P = 0.18). Conclusion: Ceftriaxone can partially inhibit microglial activation and decrease the release of IL-1β, which plays a neuroprotective role.