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使用酶的分析方法具有专一、快速、简便、无毒和便于自动化等优点,此外利用酶固定化技术又可设计“dip-stick”型分析法、点滴试验或酶电极。这些方法可在病房或急救室而不是在实验室就能对病人进行快速分析,因而特别对药物中毒的急救有用。临床化学中使用的酶是从动植物、细菌或真菌制取,其中取自动物约占60%,细菌约占30%。然而临床化学所需的某些酶如抗生素降解酶在动物体内并不存在;反之,微生物来源的
Analytical methods using enzymes are unique, fast, simple, nontoxic and easy to automate. In addition, enzyme-immobilized technologies allow the design of “dip-stick” assays, drop-on assays or enzyme electrodes. These methods allow rapid patient analysis in the ward or emergency room rather than in the laboratory and are therefore particularly useful for first aid in drug-poisoning. Enzymes used in clinical chemistry are made from animals and plants, bacteria or fungi, of which about 60% are from animals and about 30% are from bacteria. However, some of the enzymes required for clinical chemistry, such as antibiotic-degrading enzymes, do not exist in animals; conversely, microbial-derived