论文部分内容阅读
目的:通过建立脑缺血大鼠模型,观察开心解郁汤对双侧颈总动脉结扎大鼠行为学及脑血流量(CBF)的影响。方法:采用双侧颈总动脉永久结扎制备脑缺血动物模型;应用激光多普勒血流仪检测各组大鼠术后额叶、顶叶脑血流量;利用Morris水迷宫方法检测各组大鼠记忆功能。结果:与假手术对照组相比较,双侧颈总动脉永久结扎大鼠额叶、顶叶CBF明显降低,记忆功能显著下降。给予培元开郁中药3天后,与模型对照组比较,CBF加大,记忆功能障碍有所改善,但尚未达到统计学意义。结论:本实验通过建立脑缺血大鼠模型,证实了双侧颈总动脉结扎的适用性,同时说明开心解郁汤治疗双侧颈总动脉永久结扎大鼠的脑缺血有一定疗效,但尚需进行深入观察。
OBJECTIVE: To establish a rat model of cerebral ischemia and observe the effect of Kaixin Jieyu Decoction on behavior and cerebral blood flow (CBF) in bilateral common carotid artery ligation rats. METHODS: Animal models of cerebral ischemia were prepared by permanent ligation of bilateral common carotid arteries; cerebral blood flow in frontal lobe and parietal lobe were detected by laser Doppler flowmetry in each group; Morris water maze was used to detect each group. Rat memory function. RESULTS: Compared with the sham-operated control group, CBF in the frontal lobe and parietal lobe of bilateral common carotid artery ligation was significantly reduced, and the memory function was significantly decreased. After 3 days of administration of Peiyuan Kaiyu Traditional Chinese Medicine, compared with the model control group, CBF increased and memory dysfunction improved, but it did not reach statistical significance. Conclusion: This experiment establishes a rat model of cerebral ischemia and confirms the applicability of bilateral common carotid artery ligation. It also shows that Kaixin Jieyu Decoction has a certain curative effect on cerebral ischemia in bilateral permanent carotid artery ligation rats. Still need to conduct in-depth observation.