,Inhibition of ethanol-induced toxicity by tanshinone ⅡA in PC12 cells

• 作 者: Xian-fang MENG Xiao-jing ZOU Bin PENG Jing SHI Xin-min GUAN Chun ZHANG
• 机 构: Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, W
• 出 处: 中国药理学报（英文版）
• 发表日期: 2006年6期
• 关键词: apoptosis ethanol PC12 cells p53 tanshinone ⅡA

Aim: To observe the effects of tanshinone ⅡA (Tan ⅡA) on the neurotoxicity induced by ethanol in PC12 cells and to explore its protective role. Methods: PC12 cell survival was measured by MTT assay. The formation of reactive oxygen species (ROS) and lactate dehydrogenase (LDH) release were detected by 2’,7’-dichlorofluorescin (DCF) fluorescence and calorimetric method, respectively. The percentage of cell apoptosis was monitored by flow cytometry. The expression of p53 was detected by immuno-fluorescence and flow cytometry. Results: Ethanol significantly impaired the survival of PC 12 cells as demonstrated by MTT assay. Ethanol also induced significant ROS formation and increased LDH release. Pre-incubation with Tan ⅡA in the culture medium significantly reversed these changes. Ethanol caused cell apoptosis and the upregulation of p53 protein. The anti-apoptosis effects of Tan ⅡA on ethanol-induced toxicity were accompanied by the downregulation of pro-apoptotic p53 protein expression. Conclusion: Tan ⅡA can protect neurons from apoptosis and might serve as a potential therapeutic drug for neurological disorders induced by ethanol.