MicroRNA-126 inhibits the proliferation of lung cancer cell line A549

• 作 者: Xun Yang Bei-Bei Chen Ming-Hua Zhang Xin-Rong Wang
• 机 构: Respiratory Department,Central Hospital of Wuhan,
• 出 处: Asian Pacific Journal of Tropical Medicine
• 发表日期: 2015年03期
• 关键词: microRNA regulating instructions plasmid Figure Luciferase mortality downstream

Objective:To study the role of microRNA-126 in the development of lung cancer.Methods:The biological function of microRNA-126 was delected using EdU assay and CCK-8 assay:the target gene of microRNA-126 was analyzed using real time RT-PCR and Western blot assay.Results:In A549 cell line,overexpression of microRNA-126 inhibils the prolileralion rate:VEGF is the target gene of microRNA-126:microRNA-126 exerts its function via regulating VEGF protein level.Conclusions:microRNA-126 inhibits the proliferation in A549 cell line.

Objective: To study the role of microRNA-126 in the development of lung cancer. Methods: The biological function of microRNA-126 was de- toled using EdU assay and CCK-8 assay: the target gene of microRNA- 126 was analyzed using real time RT -PCR and Western blot assay. Results: In overexpression of microRNA-126 inhibils the prolileral rate: VEGF is the target gene of microRNA-126: microRNA-126 exerts its function via regulating VEGF protein level. Conclusions: microRNA- 126 inhibits the proliferation in A549 cell line.