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目的探讨白头翁总皂苷(Pulsatilla chinensis(Bunge)Regel saponins,PRS)对不同发育阶段的日本血吸虫的体外杀灭效果。方法日本血吸虫3 h和7、14 d童虫及成虫分别用0、1、5、10、20、30μg/ml PRS及30μg/ml吡喹酮(PZQ)孵育4、24、48、72 h后,观察虫体变化及状态;用扫描电镜观察30μg/ml PRS及PZQ孵育4 h内的日本血吸虫成虫的体表变化。结果PRS对日本血吸虫3 h和7、14 d童虫及成虫的体外杀灭效果呈时间和剂量依赖性。经30μg/ml PRS作用4 h后,日本血吸虫童虫及成虫全部死亡;死亡虫体颜色发暗,形态发生改变且虫体不透明。扫描电镜观察发现,PRS作用后的日本血吸虫成虫体表有不同程度损伤。结论传统中草药PRS可能对日本血吸虫感染有一定的治疗和预防作用,有希望成为新的抗血吸虫药物。
Objective To investigate the in vitro killing effect of Pulsatilla chinensis (Bunge) Regel saponins (PRS) on Schistosoma japonicum at different developmental stages. Methods Schistosoma japonicum 3 and 7, 14 d schistosomiasis and adult worms were incubated with 0, 1, 5, 10, 20 and 30 μg / ml PRS and 30 μg / ml PZQ for 4, 24, 48 and 72 h , Observe the changes and status of the parasites; observe the changes of the body surface of adult Schistosoma japonicum adults with 30μg / ml PRS and PZQ within 4 hours by scanning electron microscopy. Results The in vitro killing effect of PRS on Schistosoma japonicum 3 and 7, 14 d schistosomiasis and adults was time-and dose-dependent. After 30μg / ml PRS for 4 h, all the schistosomula and adult worms died; the color of dead worms was dark, the morphology changed and the parasites were not transparent. Scanning electron microscopy showed that the body surface of Schistosoma japonicum after PRS treatment had different degrees of damage. Conclusion The traditional Chinese herbal medicine PRS may have certain therapeutic and preventive effects on Schistosoma japonicum infection, and hopefully become a new anti-schistosome drug.