,Characterization of Xanthomonas oryzae-Responsive cis-Acting Element in the Promoter of Rice Race-S

• 作 者: Ting Yuan Xianghua Li Jinghua Xiao Shiping Wang
• 机 构: National Key Laboratory of Crop Genetic Improvement,National Center of Plant Gene Research (Wuhan),H
• 出 处: 分子植物（英文版）
• 发表日期: 2011年2期
• 关键词: Bacterial blight disease Oryza sativa UPT box Xanthomonas oryzae

The rice Xa13 gene,whose promoter harbors a UPT (up-regulated by transcription activator-like [TAL] effector) box,UPTPthXo1,plays a pivotal role in the race-specific pathogenicity caused by Xanthomonas oryzae pv. Oryzae (Xoo) strain PXO99. PXO99 causes rice disease by inducing Xa13. It is unknown,however,whether the UPTPthXo1 box is the only PXO99-responsive c/s-regulating elements in the activation of Xa13 expression. We analyzed the expression of a series of end- and site-truncated and site-mutated Xa 13 promoters in rice and the binding of PXO99 protein to the intact,partial,or site-mutated UPTPthXo1 boxes. In the Xa13 promoter,UPTPthXo1 box is the only Xoo-responsive cis-acting element that results in PXO99-induced Xa13 expression. The 5’-terminal second,third,and fourth nucleotides of the box are important for bacterial protein binding and gene activation;mutation of any one of these sites abolished PXO99-induced gene expression. Furthermore,the 3’-half of the UPTPthXo1 box is also required for protein binding and gene activation. These findings will enhance our understanding of the molecular mechanism of the interaction of rice and Xoo via UPT boxes and TAL effectors.