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应用Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交技术及放射免疫方法对人膀胱移行细胞癌组织中内皮素－１ｍＲＮＡ及内皮素活性分子的表达水平进行检测，研究了佛波酯对人膀胱移行细胞癌ＢＩＵ－８７细胞中内皮素活性分子表达水平的调节作用，并探讨了内皮素抗血清及内皮素Ａ型受体竞争性拮抗剂ＢＱ_（１２３）对ＢＩＵ－８７细胞ＤＮＡ合成的影响。结果表明，人膀胱移行细胞癌组织中内皮素－ｌｍＲＮＡ及内皮素活性分子的表达水平明显高于相应的癌旁组织，佛波酯可促进ＢＩＵ－８７细胞合成和分泌内皮素，内皮素抗血清及ＢＱ_（１２３）可抑制ＢＩＵ－８７细胞合成ＤＮＡ。提示内皮素在膀胱癌发展及ＢＩＵ－８７细胞恶性增殖中可能起重要作用。
Northern blotting and radioimmunoassay were used to detect the expression of endothelin-1 mRNA and endothelin in human bladder transitional cell carcinoma. The effects of phorbol ester on endothelin activity in human bladder transitional cell carcinoma BIU-87 cells The effect of endothelin antiserum and competitive antagonist of endothelin A receptor BQ 123 on the DNA synthesis of BIU-87 cells was also investigated. The results showed that the expression level of endothelin-1 mRNA and endothelin active molecules in human bladder transitional cell carcinoma tissues was significantly higher than that in corresponding paracancerous tissues. Phorbol ester could promote the synthesis and secretion of endothelin, endothelin antiserum And BQ_ (123) inhibited DNA synthesis in BIU-87 cells. It is suggested that endothelin may play an important role in the development of bladder cancer and the malignant proliferation of BIU-87 cells.