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目的：探讨ＩＬ６在系膜增殖性肾炎（ＭｓＰＧＮ）发病中的作用。方法：采用逆转录聚合酶链式反应方法检测６例ＭｓＰＧＮ、５例非ＭｓＰＧＮ患儿肾活检组织及４例正常肾组织中ＩＬ６ｍＲＮＡ的相对表达水平。结果：ＭｓＰＧＮ、非ＭｓＰＧＮ患儿肾组织中ＩＬ６ｍＲＮＡ表达水平（ｘ±ｓ）分别为：０４３３±０１１１和０２７１±００２３。ＭｓＰＧＮ组ＩＬ６表达水平明显高于非ＭｓＰＧＮ组（Ｐ＜００２）。４例正常肾组织中３例无ＩＬ６ｍＲＮＡ表达，余１例表达水平为０１９２。中度ＭｓＰＧＮ和轻度ＭｓＰＧＮ表达水平分别为：０４９１±００８４和０３１７±００１８，二者比值为１５∶１，ＭｓＰＧＮ伴肾小球局灶节段硬化和不伴硬化组其表达水平分别为０５１６±００８３和０３５０±００５８，二者比值为１４７∶１。另采用生物活性法测定ＭｓＰＧＮ患儿尿中ＩＬ６活性水平，发现尿中ＩＬ６活性与肾组织中ＩＬ６ｍＲＮＡ的表达水平明显正相关（ｒ＝０９７７，Ｐ＜０００１）。结论：ＩＬ６可能与ＭｓＰＧＮ发病机理有关，ＩＬ６可能参与ＭｓＰＧＮ系膜增殖和硬化，尿中ＩＬ６活性测定可反映肾组织局部ＩＬ６ｍＲＮＡ的表达情况
Objective: To investigate the role of IL6 in the pathogenesis of mesangial proliferative glomerulonephritis (MsPGN). Methods: The relative expression levels of IL6 mRNA in 6 cases of MsPGN, 5 cases of non-MsPGN in renal biopsy and 4 cases of normal renal tissues were detected by reverse transcriptionpolymerase chain reaction. Results: The expression of IL6mRNA in kidney tissues of MsPGN and non-MsPGN patients were (x ± s) 0433 ± 0111 and 0271 ± 0023, respectively. MsPGN group IL 6 expression was significantly higher than non-MsPGN group (P <0 02). 4 cases of normal renal tissue in 3 cases without IL 6 mRNA expression, the remaining 1 cases the expression level was 0192. The moderate MsPGN and mild MsPGN expression levels were 0491 ± 0084 and 0317 ± 0018, the ratio of the two was 15: 1, MsPGN with focal segmental sclerosis of glomerulus and not The expression levels in patients with sclerotic lesions were 0516 ± 0083 and 0350 ± 0058, respectively, with the ratio of 147:1. In addition, the activity of IL-6 in urine of MsPGN children was determined by bioactivity assay. The activity of IL-6 in urine was positively correlated with the expression of IL-6 mRNA in renal tissues (r = 0.977, P <0.001) . Conclusion: IL 6 may be related to the pathogenesis of MsPGN, IL 6 may be involved in MsPGN mesangial proliferation and sclerosis, urinary IL 6 activity can reflect the local tissue expression of IL 6 mRNA