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对照和仅用6-BA(无Ca~(2+))处理的原生质体的体积均无变化。含钙培养液中的原生质体经6-BA处理后10min~(45)Ca~(2+)积累明显增多,15min后开始膨大;处理30min时~(45)Ca~(2+)积累最多,此时原生质体的膨大效应最好;随后~(45)Ca~(2+)积累和膨大效应逐渐下降。两者的时间进程十分相似。K~+、Zn~(2+)、Ba~(2+)、Mg~(2+)等可在不同程度上代替Ca~(2+)的作用。EGTA、verapamil和LaCl_3处理均可使原生质体~(45)Ca~(2+)累积降到对照的水平,膨大效应完全消失。表明Ca~(2+)可能在6-BA诱导原生质体膨大的过程中起着重要作用。
There was no change in the volume of the control and protoplasts treated with 6-BA alone (Ca 2+ -free). The protoplasts in calcium-containing culture medium accumulated significantly after 10 min ~ (45) Ca ~ (2+), and began to enlarge after 15 min. The accumulation of Ca ~ (45) Ca ~ (2+) At this time protoplast expansion effect is the best; then ~ (45) Ca ~ (2+) accumulation and expansion effect gradually decreased. The time course of the two is very similar. K ~ +, Zn ~ (2 +), Ba ~ (2 +), Mg ~ (2+) can replace Ca ~ (2+) to some extent. EGTA, verapamil and LaCl_3 could both reduce the accumulation of Ca ~ (45) in protoplasts to the level of control, and the expansion effect completely disappeared. This indicated that Ca 2+ might play an important role in the process of 6-BA induction of protoplast enlargement.