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1984～1985年,从62个水稻品种中筛选出南京11、大艮鱼、金刚、奥羽244和滇榆1号共5个慢瘟性较强的品种和丽江新团黑谷与朝6早两个速瘟性品种作为供试材料,进行了慢瘟性及其机制的研究。对构成慢瘟性的组分分析发现,慢瘟性与侵染频率和单病斑产孢量星负相关(r=0.98和r=—0.81),但与潜育期不相关。生理生化测定表明,慢瘟性品种的硅含量、剑叶表皮硅化细胞数、酚类和类黄酮物质含量均比速瘟性品种的高。稻叶中提取的酚类物质经纸层析分离,出现5个蓝色斑,其中已确定的有阿魏酸、对一香豆酸和绿原酸。室内测定这3种酚酸在10~(-3)M浓度时对稻瘟菌菌丝和孢子芽管的生长具有明显的抑制作用。组织学观察发现,接种40小时后,侵入慢瘟性品种体内的菌丝的生长受抑制,其扩展范围仅限于受侵的单个细胞,且未发现此细胞产生颗粒状物质和过敏性反应,而在速疲性品种中,菌丝生长迅速而繁茂,并扩展到邻近的几层细胞中。
From 1984 to 1985, 5 rice varieties with strong blast resistance, Nanjing 11, Gorgon fish, King Kong, Ouyu 244 and Dian Yu 1, were selected from 62 rice varieties, A speedy variety as a test material, carried out a study of the mechanism of chronic pandemic. Analysis of the components that make up the slow pandemic showed that there was a negative correlation between the frequency of chronic blast and infection and the sporulation of single disease (r = 0.98 and r = -0.81), but not with the latent period. Physiological and biochemical tests showed that the content of silicon, the silicified cell number of epidermis, the contents of phenols and flavonoids in the two kinds of blast-resistant cultivars were higher than that of the two blast-cultivars. The phenolic compounds extracted from rice leaves were separated by paper chromatography and five blue spots appeared, of which ferulic acid, citric acid and chlorogenic acid were identified. In vivo determination of these three phenolic acids in the 10 ~ (-3) M concentration on Magnaporthe grisea and spore germination tube significantly inhibited. Histological observations revealed that the growth of mycelia invaded into the slow-blasting cultivars was inhibited 40 hours after inoculation, and its expansion was limited to the single cells invaded and no granulocytes and anaphylactoid reactions were found, whereas In the fast-growing variety, mycelium grows quickly and lumps and spreads to nearby layers of cells.