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目的探讨子宫颈癌组织中抗凋亡基因bcl-2、癌基因c-erbB2蛋白表达,以及与子宫颈癌发生、发展及预后的关系。方法应用免疫组化S-P法检测24例宫颈鳞癌、20例宫颈上皮内瘤样变(cervicalintraepithelialneoplasia,CIN)和10例正常宫颈组织中bcl-2、c-erbB2的表达情况。结果bcl-2、c-erbB2在宫颈鳞癌、CIN和正常宫颈组织中阳性率分别为75%(18/24)、58.3%(14/24);60%(12/20)、40%(8/20);20%(2/10)、0%(0/10)。bcl-2在鳞癌、CIN与正常组织中的表达有显著性差异(P<0.001),而鳞癌组与CIN组之间差异无显著性(P>0.05)。c-erbB2在鳞癌、CIN中的表达与正常组织相比差异有显著性(P<0.05)。bcl-2的表达与组织学类型、病理分级及临床分期有关(P<0.05)。c-erbB2表达与组织学类型、病理分级及临床分期无关(P>0.05)。结论在宫颈上皮癌早期可能发生bcl-2的过表达,c-erbB2的过表达是细胞恶变的标志之一。
Objective To investigate the expression of anti-apoptotic gene bcl-2 and c-erbB2 protein in cervical cancer and its relationship with the occurrence, development and prognosis of cervical cancer. Methods The expressions of bcl-2 and c-erbB2 in 24 cases of cervical squamous cell carcinoma, 20 cases of cervical intraepithelial neoplasia (CIN) and 10 cases of normal cervical tissue were detected by immunohistochemical S-P method. Results The positive rates of bcl-2 and c-erbB2 in cervical squamous cell carcinoma, CIN and normal cervical tissue were 75% (18/24), 58.3% (14/24), 60% (12/20), 40% 8/20); 20% (2/10), 0% (0/10). The expression of bcl-2 was significantly different between squamous cell carcinoma and CIN tissues (P <0.001), while there was no significant difference between squamous cell carcinoma and CIN (P> 0.05). The expression of c-erbB2 in squamous cell carcinoma and CIN was significantly lower than that in normal tissues (P <0.05). The expression of bcl-2 was correlated with histological type, pathological grade and clinical stage (P <0.05). The expression of c-erbB2 was not related to histological type, pathological grade and clinical stage (P> 0.05). Conclusion The overexpression of bcl-2 may be an early stage of cervical cancer. The overexpression of c-erbB2 is one of the markers of cell malignant transformation.