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目的利用失重飞行提供的微重力环境验证多坝式空间细胞培养室的设计参数。方法设计了三坝式和五坝式两种结构的插片细胞培养室,在失重飞机抛物线飞行中,通过研究培养室内流场形态、液流更换效率及流速对室内细胞的影响,对其在微重力下的适用性进行了验证。结果三坝或五坝式结构均能在室内隔离形成有效液流通路,确保微重力环境下培养室内溶液的有效更替;插片式设计在方便取出细胞样品用于后续分析的同时,可将细胞培养片固定在培养室底部防止刮蹭细胞;微重力下50μL/min和150μL/min两种换液流速均不会损伤细胞。结论综合考虑空间细胞培养的高容积利用率及短操作时间等需求,插片式三坝细胞培养室结构及150μL/min的换液流速更符合应用要求。
Objective To verify the design parameters of a multi-dam space cell culture chamber by using the microgravity environment provided by weightless flight. Methods Three-bar and five-bar plug-in cell culture chambers were designed. In the parabolic flight of weight-loss aircraft, by studying the influence of the shape of the flow field in the chamber, the flow changing efficiency and the flow rate on the indoor cells, The applicability under microgravity was verified. Results Three dams or five dams can be isolated in the room to form an effective flow path to ensure that the micro-gravity environment to cultivate the effective replacement of indoor solution; plug-in design in the easy removal of the cell sample for subsequent analysis at the same time, cells The culture plates were fixed on the bottom of the culture chamber to prevent scratching the cells; under the microgravity, the flow rates of 50 μL / min and 150 μL / min did not damage the cells. Conclusion Considering the needs of high volume utilization and short operation time of space cell culture, the structure of plug-in Sanba cell culture chamber and the flow rate of 150 μL / min are more suitable for the application.