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研究了金龟子绿僵菌菌株M a83液体培养最佳营养及环境条件,为无纺布培养提供良好的液体种子。以生物量为指标,通过液体摇瓶试验确定了绿僵菌M a83菌株液体种子培养参数,对不同的氮源、碳源及其浓度和初始pH进行单因素分析。结果表明,绿僵菌M a83菌株液体种子培养最佳营养条件为4%黄豆粉,2%白砂糖,0.5%MgSO4.7H2O,0.05g/LKC l,0.1g/L FeSO4.7H2O,1.0g/L KH2PO4,pH6.3~6.5。70L发酵罐培养,干生物量第三天可达35g/L,显著优于筛选前培养基(SDA,28g/L,a=0.05)。
The optimum nutrient and environmental conditions for the liquid culture of Metarhizium anisopliae strain M a83 were studied, which provided good liquid seeds for the culture of non-woven fabrics. Based on biomass, liquid culture parameters of M a83 strain of Metarhizium anisopliae were determined by liquid shake flask test, and the single factor analysis of different nitrogen source, carbon source, its concentration and initial pH were carried out. The results showed that the optimal nutrient conditions for liquid seed culture of M a83 strain of Metarhizium anisopliae were 4% soybean flour, 2% sugar, 0.5% MgSO4.7H2O, 0.05g / L KCl, 0.1g / L FeSO4.7H2O, 1.0g / L KH2PO4, pH6.3 ~ 6.5.70L fermenter. The dry biomass reached 35g / L on the third day, which was significantly better than the pre-screening medium (SDA, 28g / L, a = 0.05).