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目的研究犬全缺血后浅低温对脑内源性神经保护的影响。方法 15只健康杂种犬随机分为3组:手术对照组(A 组,n=4只),缺血再灌注组(B 组,n=5只),浅低温治疗组(C 组,n=6只)。A 组动物完成麻醉和手术后心脏不停跳观察8 h。B 组和 C 组动物心脏停跳18 min,心脏复苏成功后,B 组动物观察8 h,C 组动物接受浅低温[(34±0.5)℃]治疗。实验结束时开颅取右顶叶脑皮质供测超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)含量和免疫组织化学法显示小白蛋白(PV)和热休克蛋白70(HSP_(70))阳性神经元。结果 B 组动物脑内 GSH、GSH-Px、T-SOD、Mn-SOD、Cu-ZnSOD、PV 阳性神经元数量显著低于 A 组(P<0.05或 P<0.01),而 HSP_(70)-LI 阳性神经元数量明显高于 A 组[(15.6±3.7)vs(5.5±2.1),P<0.05]。C 组动物脑内 GSH、T-SOD、Cu-ZnSOD、PV 阳性神经元和 HSP_(70)-LI 阳性神经元数量明显高于 B 组 PV-I 神经元密度:(34.7±6.3)vs(23.8 5.3),P<0.05;HSP_(70)-LI 阳性神经元密度:(27.1±4.9)vs(15.6±3.7),P<0.05。结论脑内内源性神经保护作用增强可能是浅低温治疗脑缺血的机制之一。
Objective To study the effect of mild hypothermia on endogenous neuroprotection after brain ischemia in dogs. Methods Fifteen healthy mongrel dogs were randomly divided into three groups: operation control group (n = 4), ischemia reperfusion group (n = 5), mild hypothermia group (n = 6). A group of animals completed anesthesia and heart beating after surgery observed 8 h. Animals in groups B and C were beated for 18 min. After successful cardiac resuscitation, animals in group B were observed for 8 h. Animals in group C received mild hypothermia [(34 ± 0.5) ° C]. At the end of the experiment, the content of superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-Px) and immunohistochemistry Small albumin (PV) and heat shock protein 70 (HSP 70) positive neurons. Results The number of GSH, GSH-Px, T-SOD, Mn-SOD, Cu-ZnSOD and PV positive neurons in group B were significantly lower than those in group A (P <0.05 or P < The number of positive neurons in LI group was significantly higher than that in A group [(15.6 ± 3.7) vs (5.5 ± 2.1), P <0.05]. The numbers of GSH, T-SOD, Cu-ZnSOD, PV positive neurons and HSP70-LI positive neurons in group C were significantly higher than those in group B (34.7 ± 6.3) vs (23.8 5.3), P <0.05; HSP70 (70) -LI positive neurons density: (27.1 ± 4.9) vs (15.6 ± 3.7), P <0.05. Conclusion The enhancement of endogenous neuroprotection in the brain may be one of the mechanisms of hypothermia in the treatment of cerebral ischemia.