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目的探讨A(或B)型新生儿在新生儿溶血病放散试验中放散出抗-B(或抗-A)的原因。方法采用体外模拟实验:取O型孕妇血浆2 m L经2-Me破坏后分别与A(或B)型红细胞2 mL 37℃孵育1 h,分离红细胞和血浆,分离出的血浆再用2 m L A(或B)型红细胞完成2次吸收,检测红细胞吸收前后血浆的Ig G抗-A和抗-B效价;对吸收后红细胞做56℃热放散,检测放散液中的抗-A和抗-B。结果 O型血浆用A型红细胞2次吸收后,抗-A效价下降至0,抗-B效价下降1-4个滴度;O型血浆用B型红细胞2次吸收后,抗-B效价下降至0,抗-A效价下降1-2个滴度;第1次吸收后的Ac和Bc放散液均检出抗-A和抗-B,第2次吸收试验后的Ac放散液只检出了抗-B,Bc放散液只检出了抗-A。结论新生儿溶血病放散试验A(或B)型新生儿放散出的抗-B(或抗-A)为低效价抗-AB。
Objective To investigate the causes of A (or anti-A) in A (or B) newborn during the newborn hemolytic disease test. Methods The in vitro experiments were performed in this experiment. The plasma of 2-Me was taken from 2-Me-damaged pregnant women and incubated with 2 mL of A (or B) erythrocytes at 37 ℃ for 1 h respectively. The separated plasma and plasma were separated by 2 m The LA (or B) type erythrocytes completed two absorptions, and the plasma IgG anti-A and anti-B titers were measured before and after erythrocyte absorption. The absorbed erythrocytes were subjected to heat dissipation at 56 ° C to detect the anti-A and anti- -B Results After O-type plasma was twice absorbed by type A red blood cells, the anti-A titer decreased to 0 and the anti-B titer decreased by 1-4 titers. After O-type plasma was absorbed twice by type B erythrocytes, anti- The titer dropped to 0 and the anti-A titer decreased by 1-2 titers. Both Ac-B and anti-B were detected after the first absorbance of Ac and Bc, and Ac after the second absorbance test Only anti-B was detected in the solution, and only anti-A was detected in the Bc effusion. Conclusions The anti-B (or anti-A) released from newborn hemolytic disease test A (or B) is a low-potency anti-AB.