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应用检测特异性CTL活性的方法检测乙脑活疫苗的细胞免疫应答 ,并以灭活疫苗作对照。结果显示活疫苗免疫小鼠后诱导的CTL均值为 79 2 % ,较灭活疫苗 2 9%高 5 0 2 % ,表明活疫苗具有较强的特异性CTL活性。用同批活疫苗免疫小鼠后其中和抗体效价为 1:5 ,而同批的灭活疫苗为 1:2 0 ,活疫苗的免疫效力 (ID5 0 ) 3 6× 10 6(ml)显著高于灭活疫苗的 4 0× 10 - 4 ～ 4 2× 10 4(ml)。表明乙脑活疫苗诱导的免疫应答中细胞免疫在保护效力中占有重要地位。
The detection of the specific CTL activity of the method of detection of JN live vaccine cellular immune response and inactivated vaccine as a control. The results showed that the average CTL induced by live vaccine in mice was 79 2%, which was 52.02% higher than that of inactivated vaccine, indicating that the live vaccine had strong specific CTL activity. The neutralizing antibody titer was 1: 5 when the mice were immunized with the same live vaccine, while the inactivated vaccine of the same batch was 1: 20. The immune efficacy (ID50) of the live vaccine was 36 × 10 6 (ml) Higher than inactivated vaccine 4 0 × 10 -4 ~ 4 2 × 10 4 (ml). This suggests that cellular immunity in the immune response induced by JE live vaccine plays an important role in the protective efficacy.