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目的建立两次打击大鼠所致的多器官功能障碍综合征(MODS)的动物模型,研究动物各器官代偿与失代偿的反应过程。方法选择清洁级SD大鼠,随机分为正常对照组(A组,32只),出血+腹腔注射内毒素组(B组,42只),出血组(C组,42只),腹腔注射内毒素组(D组,42只)。在实验的第8、24、48、72小时随机抽取每组大鼠各8只,观察各组大鼠的症状、体征、死亡率;血清内毒素血症阳性率(鲎试验);各脏器功能指标[外周血白细胞总数(WBC)、动脉血氧分压(PaO2)、丙氨酸转氨酶(ALT)、血尿素氮(BUN)、血肌酸激酶(CK)]及重要脏器的病理形态学变化。结果在实验8～24h,B组大鼠的症状、体征与功能指标(WBC、PaO2、ALT、BUN、CK)均有明显变化,与A组、C组、D组差异有显著性;其肺、肝、肾、心、脾及小肠等重要脏器病理改变明显。在48～72h,存活大鼠的症状、体征、功能指标及重要脏器病理变化逐步恢复;血清鲎试验阳性率为16.7%。MODS的发生率45.2%,总死亡率为21.4%。结论以失血加腹腔内注射内毒素的方法,可成功复制大鼠MODS模型,方法简便,能较好地反映大鼠对创伤和感染因素表现出器官功能由代偿到失代偿的反应过程。
Objective To establish two animal models of combating multiple organ dysfunction syndrome (MODS) caused by rats and to study the response process of various organ compensations and decompensation in animals. Methods SD rats were randomly divided into normal control group (A group, 32 rats), hemorrhage + intraperitoneal injection of endotoxin group (B group, 42 rats), hemorrhage group (C group, 42 rats) Toxin group (D group, 42). At the 8th, 24th, 48th, and 72th hours of the experiment, 8 rats in each group were randomly selected to observe the symptoms, signs and mortality of the rats in each group. The positive rate of serum endotoxemia (鲎 test) Function index (WBC, PaO2, ALT, BUN, CK) and pathological changes of vital organs Learning changes. Results The symptoms, signs and function indicators (WBC, PaO2, ALT, BUN, CK) in group B were significantly different from those in group A, C and D at 8 to 24 hours. , Liver, kidney, heart, spleen and other important organs of the small intestine pathological changes. At 48 ~ 72h, the symptoms, signs, function indexes of the surviving rats and the pathological changes of vital organs were gradually recovered. The positive rate of serum 鲎 test was 16.7%. The incidence of MODS was 45.2% and the total mortality was 21.4%. Conclusion The model of MODS can be successfully replicated by the method of blood loss plus intraperitoneal injection of endotoxin. The method is simple and can reflect the response process of compensatory to decompensated organ function of traumatic and infectious agents in rats.