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目的研究复方鳖甲方治疗博莱霉素诱导的实验大鼠特发性肺纤维化(Id iopathic pulmonaryfibrosis IPF)的超微结构变化。方法180只SD雄性大鼠随机分为6组,假手术对照组、模型组、阳性药物对照组及复方鳖甲方高、中、低剂量组。在药物治疗的第7、14、28天,每次每组随机抽取大鼠10只麻醉后取材,常规固定制成切片,光镜观察其结构和胶原变化。通过图像分析系统,测定支气管周围及肺泡透光区、致密区的平均光密度。常规制造电镜切片观察其超微结构。结果光镜观察显示复方鳖甲方可降低肺纤维化程度。图像分析表明:假手术组和模型组在7、14、28 d之间光密度无显著性差异;其余各组三阶段之间有不同差异。电镜观察,复方鳖甲方各组在7、14、28 d胶原纤维含量均明显少于模型组和阳性药物组,基膜区域性折叠、扭曲或缺损。细胞外基质沉积、呼吸膜增厚等现象也明显好于模型组和阳性药物组。结论复方鳖甲方有明显抑制实验大鼠IPF胶原纤维增生的作用。
Objective To study the ultrastructural changes of Compound IBG in the treatment of bleomycin-induced Idiopathic pulmonary fibrosis (IPF) in experimental rats. Methods 180 SD male rats were randomly divided into 6 groups: sham-operated control group, model group, positive drug control group, and high-, medium-, and low-dose group. On the 7th, 14th, and 28th days of drug treatment, 10 rats were randomly selected from each group to take anesthesia after being anesthetized and routinely fixed to make sections. The structure and collagen changes were observed by light microscopy. Through the image analysis system, the average optical density around the bronchi and in the translucent and dense areas of the alveoli was measured. Electron microscopy was routinely fabricated to observe its ultrastructure. RESULTS: Light microscopy showed that compound Fangjiafang can reduce the degree of pulmonary fibrosis. Image analysis showed that there was no significant difference in optical density between the sham operation group and the model group at 7, 14, and 28 days; there were differences between the rest of the three groups. Electron microscopy showed that the contents of collagen fibers at the 7th, 14th, and 28th days of the compound Fufangjiajiao group were significantly lower than those of the model group and the positive drug group, and the basement membrane was folded, distorted or defected. Extracellular matrix deposition and thickening of the respiratory membrane were also significantly better than the model group and the positive drug group. Conclusion Compound Fufangjiafang can obviously inhibit the collagen fibrosis of IPF in experimental rats.