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目的探讨少突胶质细胞相关microRNA(m iRNA)对中枢神经系统神经前体细胞增殖的作用。方法利用D icer1 loxp/loxp基因型小鼠和Olig1-Cre品系小鼠杂交获得miRNA加工酶Dicer基因条件性敲除小鼠,再用免疫荧光染色和B rdU标记等技术检测这种Dicer条件性敲除小鼠大脑和小脑中神经前体细胞的增殖,以及脊髓中新生神经元的变化情况。结果 Dicer条件性敲除小鼠大脑中神经前体细胞增殖加快,而小脑中增殖细胞数目较正常小鼠减少(12%,P<0.05)。此外脊髓中神经元的数目并未受到影响。结论少突胶质细胞相关m iRNA对中枢神经系统的胶质细胞的发生和增殖具有时空特异性或差异性调节作用。
Objective To investigate the effect of oligodendrocyte-associated miRNAs (miRNAs) on the proliferation of neural precursor cells in the central nervous system. Methods Dicer gene knockout mice were obtained by crossing D icer1 loxp / loxp mice and Olig1-Cre mice. The Dicer conditional knockout mice were detected by immunofluorescence staining and B rdU labeling Except for the proliferation of neural progenitor cells in the mouse brain and cerebellum, and changes in the neonate neurons in the spinal cord. Results Proliferation of neural precursor cells was accelerated in the brain of Dicer knockout mice, while the number of proliferating cells in cerebellum was decreased compared with that of normal mice (12%, P <0.05). In addition, the number of neurons in the spinal cord has not been affected. Conclusion The oligodendrocyte-associated miRNAs have a spatiotemporal-specific or differential regulation of glial cells in the central nervous system.