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一氧化氮(NO)是一种具有高度反应性和各种生理活性的分子。一氧化氮合成酶有三种同功酶:内皮细胞性(eNOS)、神经细胞性(nNOS)和诱导性(iNOS)。在肿瘤微循环中,NO在维持肿瘤血流和营养方面可能起作用。作者提出这样假说:①来自于肿瘤血管内皮细胞以及(或)瘤细胞的内源性NO增加和(或)维持肿瘤血流、减少白细胞-内皮细胞间反应以及增加血管通透性;②外源性NO能通过血管扩张以增加肿瘤血流、减少白细胞-内皮细胞间反应;③NO的产生以及组织对NO反应是肿瘤依赖性。为了验证上述假说以及确定NO在肿瘤中作用,作者应用NOS抑制剂以及外源性NO观察肿瘤血流、血管管径、白细胞-内皮细胞间相互作用以及血管通透性的变化。
Nitric oxide (NO) is a molecule with high reactivity and various physiological activities. Nitric oxide synthase has three isoenzymes: endothelial cell (eNOS), neuronal (nNOS) and inducible (iNOS). In the tumor microcirculation, NO may play a role in maintaining tumor blood flow and nutrition. The authors proposed this hypothesis: 1 Increased endogenous NO from tumor vascular endothelial cells and/or neoplastic cells and/or maintenance of tumor blood flow, reduction of leukocyte-endothelial cell response, and increased vascular permeability; 2 Nitric oxide can increase tumor blood flow and decrease leukocyte-endothelial cell reaction through vasodilation; 3NO production and tissue response to NO are tumor-dependent. To verify the above hypothesis and determine the role of NO in tumors, the authors used NOS inhibitors and exogenous NO to observe changes in tumor blood flow, vascular diameter, leukocyte-endothelial cell-cell interaction, and vascular permeability.