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为探讨菌株xj063-1的最优发酵条件,以菌体生物量和对病原菌的抑制率为指标,采用单因素方法对菌株的发酵条件进行优化,并将优化的发酵液在离体枣果上进行防效测定。结果表明,当装液量为50 m L时,拮抗菌株生物量OD600为1.987,对病原菌的抑制率为67.1%;接种量为装液量体积的4%时,生物量OD600为1.573,对病原菌的抑制率为59.7%;初始p H 7.0时,其OD600为1.905,对病原菌的抑制率为65.6%;温度在30℃、转速180 r/min时,OD600分别为1.969和1.982,对病原菌的抑制率分别为62.2%和61.2%。拮抗菌株优化后的发酵液通过预防法处理后的枣果的病斑面积为4 mm2,对照发病面积为121 mm2,抑制率为96.7%;通过治疗法处理后的枣果的病斑面积为12.25 mm2,抑制率为89.9%,表明菌株xj063-1具有较好的生防潜力。
In order to investigate the optimal fermentation conditions of strain xj063-1, the fermentation conditions of the strains were optimized by single factor method using the biomass of the cells and the inhibition rate of the pathogenic bacteria as an indicator, and the optimized fermentation broth was isolated on jujube Anti-efficacy determination. The results showed that when the liquid volume was 50 m L, the OD600 of the antagonistic strain was 1.987, and the inhibition rate to the pathogen was 67.1%. When the inoculum size was 4% of the volume of the liquid, the OD600 of the biomass was 1.573, The inhibition rate was 59.7%; OD600 was 1.905 at initial p H 7.0, and the inhibition rate to pathogen was 65.6%; OD600 was 1.969 and 1.982 at temperature of 30 ℃ and rotation speed of 180 rpm Rates were 62.2% and 61.2% respectively. Antagonistic strains of the optimized fermentation broth after treatment of jujube fruit lesions area of 4 mm2, the control incidence of 121 mm2, the inhibition rate of 96.7%; treated by treatment of jujube fruit spot area of 12.25 mm2, the inhibition rate was 89.9%, indicating that the strain xj063-1 has better biocontrol potential.