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目的探索血浆内血管活性物质和白细胞浸润在大鼠局灶性脑缺血再灌损伤中是否影响力不同及三乙酰莽草酸(TSA)的保护作用。方法线栓法制备大鼠大脑中动脉缺血90 min再灌注3 ~48 h模型。分别于缺血开始和缺血60 min时给予TSA 50~200 mg·kg-1ig。分别用荧光分光光度法和放射免疫法测定血浆5-羟色胺(5-HT)和血栓烷素B2(TXB2)含量,化学法测定脑皮层中髓过氧化物酶(MPO)活性。结果大鼠脑缺血再灌注3 ~24 h时血浆5-HT和TXB2含量及脑MPO活性呈时间依赖性升高,48 h后5-HT, TXB2含量降至假手术组水平,而MPO活性仍明显高于未缺血侧脑皮层。TSA(100和200 mg·kg-1)可显著抑制缺血90 min再灌注24 h时血浆5-HT和TXB2含量及脑MPO活性增高。结论大鼠局灶性脑缺血再灌注过程中血浆中血管活性物质量和脑组织中MPO活性表现出不同的时相变化,并且对脑损伤影响力不同,TSA可有效保护缺血脑组织。
Objective To explore whether plasma vasoactive substances and leukocyte infiltration in rats with focal cerebral ischemia-reperfusion injury whether the influence of different and triacetyl shikimic acid (TSA) protective effect. Methods The rat middle cerebral artery occlusion (MCAO) model was established by reperfusion for 90 min and reperfusion for 3 ~ 48 h. TSA 50 ~ 200 mg · kg-1ig was given at the beginning of ischemia and 60 min after ischemia, respectively. Plasma 5-hydroxytryptamine (5-HT) and thromboxane B2 (TXB2) contents were determined by fluorospectrophotometry and radioimmunoassay, respectively. The activity of myeloperoxidase (MPO) in the cortex was measured by chemical method. Results The content of 5-HT and TXB2 and the MPO activity in brain increased in a time-dependent manner at 3 ~ 24 h after cerebral ischemia-reperfusion in rats. The levels of 5-HT and TXB2 decreased to the level of sham operation group after 48 h, while the MPO activity Still significantly higher than non-ischemic cortex. TSA (100 and 200 mg · kg-1) significantly inhibited the increase of plasma 5-HT and TXB2 content and brain MPO activity at 90 min after reperfusion for 90 min. Conclusions The amount of vasoactive substances in plasma and the activity of MPO in brain tissue during focal cerebral ischemia-reperfusion in rats show different time-phase changes, and have different effects on brain injury. TSA can effectively protect ischemic brain tissue.