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目的采用火焰吸收原子分光光度法(FAAS)检测全身照射小鼠血清中不同化学形态铜元素含量,探究辐照后小鼠血清铜元素含量变化机制及其作为生物剂量计的可行性。方法昆明种雄性小白鼠78只,鼠龄5周,体质量20~22 g,清洁级。按随机数字表法,根据照射剂量0、4.0、6.0、8.0 Gy分为对照组与照射组,再根据照射时间1、4、10、24 h共分为13组,每组6只。照射组1~4亚组给予4.0 Gy剂量照射,照射时间为1.5 min;5~8亚组给予6.0 Gy剂量照射,照射时间为2 min 15 s;9~12亚组给予8.0 Gy剂量照射,照射时间为3 min;对照组不给予照射。从小鼠眼眶取血后分离血清,用乙醇蛋白沉淀法分离血清中不同化学形态铜元素,火焰原子吸收光谱法测量血清铜质量浓度。采用单因素方差分析法对所得数据进行处理。结果不同剂量照射小鼠游离态血清铜质量浓度24 h内变化无统计学意义(P>0.05);照射后24 h内小鼠结合态血清铜质量浓度变化有统计学意义(P<0.05),其中照射剂量为4.0 Gy、6.0 Gy时变化趋势为先上升后下降,峰值分别位于4 h、10 h处;照射剂量为8.0 Gy时变化趋势为单调上升。照射后24 h小鼠结合态血清铜质量浓度、血清铜总质量浓度与辐照剂量呈良好线性关系,直线方程为Y=0.047 3X+0.365 4、Y=0.048 6X+0.530 9(R2=0.972 6、0.982 6)。结论照射小鼠体内血清铜元素含量发生变化,主要体现在血清中参与细胞保护与修复等生理活动的结合态铜元素含量变化;生物体在调控结合态铜元素含量变化来进行细胞保护修复的同时并未产生细胞毒性;照射后24 h小鼠结合态血清铜质量浓度、血清铜总质量浓度具备发展成为新型生物剂量潜力。
Objective To detect the contents of copper (Cu) in serum of mice exposed to whole body irradiation by flame atomic absorption spectrometry (FAAS) and to explore the mechanism of copper content in mice after irradiation and its feasibility as a biological dosimeter. Methods A total of 78 Kunming male mice aged 5 weeks and weighing 20-22 g were cleansed. According to the random number table method, according to the irradiation dose 0,4.0,6.0,8.0 Gy divided into control group and irradiation group, then according to the irradiation time 1,4,10,24 h is divided into 13 groups, 6 in each group. Irradiation dose of 4.0 Gy was given in irradiation subgroups 1 to 4 for 1.5 min, irradiation dose of 6.0 Gy was given in subgroups 5 to 8 for 2 min 15 s, irradiation dose of 8.0 Gy was given in subgroups 9 to 12, Time was 3 min; control group did not give irradiation. Serum samples were collected from the orbital of mice and the different chemical forms of copper were separated by ethanol precipitation. The concentration of copper in serum was measured by flame atomic absorption spectrometry. The data were processed using one-way ANOVA. Results There was no significant difference in the concentration of free copper in 24 h after exposure to different doses of irradiation (P> 0.05). The concentration of copper in the bound serum of mice was significantly increased within 24 h after irradiation (P <0.05) At the dose of 4.0 Gy and 6.0 Gy, the change trend first increased and then decreased, and the peak values were at 4 h and 10 h respectively. When the irradiation dose was 8.0 Gy, the change tendency monotonically increased. The 24 hours after irradiation, the concentration of serum copper in the combined state showed a good linear relationship with the dose of radiation. The linear equation was Y = 0.047 3X + 0.365 4, Y = 0.048 6X + 0.530 9 (R2 = 0.972 6 , 0.982 6). Conclusion The changes of serum copper levels in irradiated mice are mainly reflected in the changes of the content of bound copper in the serum involved in physiological activities such as cell protection and repair. In the meantime, Did not produce cytotoxicity; 24 h after irradiation, the concentration of copper in serum of mice combined with the total serum copper concentration has the potential to develop into a new biological dose.