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研究对象是53例不吸烟的健康人,年龄19～40岁。仔细检查口腔,在上皮无角化层的唇颊粘膜以及上皮有生理角化层的龈和硬腭粘膜,取印迹涂片,除普通染色以外,利用特殊的组织化学技术,包括Danielli反应、Feulgen反应(核酸染色);利用溴酚蓝(pH8.2)、亮绿(pH8.2)和氮化黑(pH8.1)染色,检查细胞的阳离子蛋白;用Barnett.Seliyman法(1952)测定胱氨酸巯基,用М.Г.Щубин法检查蛋白的二硫基,用Goldberg(1962)法检查酸性磷酸酶,用Kylenkamp(1977)法检查酸性非特异性酯酶。 结果发现健康人口腔粘膜印迹涂片,可以识别无核的上皮鳞屑和表层有核细胞,有核细胞的胞浆可有或无颗粒。众所周知,角化上皮区有4层:基底层、棘层、粒层和角化层,而在涂片上只看到无核鳞屑和中间型细胞,胞浆含颗粒的细胞很少;而非角化区粘膜涂片
The subjects were 53 non-smoking healthy people, aged 19 to 40 years old. Careful examination of the oral cavity, the labial mucosa of the keratinized epithelial layer, the mucosa of the gingival and palatal palate of the keratinized layer of the epithelium, blotting smears were taken, and besides the ordinary staining, special histochemical techniques including Danielli reaction, Feulgen reaction (Nucleic acid staining); Cationic protein was examined by staining with bromophenol blue (pH 8.2), brilliant green (pH 8.2) and nitric oxide black (pH 8.1); Cystamine was assayed by Barnett.Seliyman method (1952) Acid sulfhydryl groups were examined for the disulfide group of the protein by the method of M. G. Fernandes, acidic phosphatase was assayed by the method of Goldberg (1962) and acidic non-specific esterase was examined by the method of Kylenkamp (1977). The results found that healthy people oral mucosa blot smears, can identify non-nuclear epithelial scales and superficial nuclear cells, nucleated cells with or without the cytoplasm. It is well-known that the keratinized epithelium has 4 layers: basal layer, stratum spinosum, granular layer and keratinized layer, while in the smear only see the non-nuclear scaly and intermediate type cells, the cytoplasm contains little granule cells; Keratosis mucosal smear