菜籽饼粕中有毒代谢物的改进测定方法(英文)

• 作 者: 韩华琼 李伟格 谢发明 范理 杨向千 许慧嘉
• 机 构: 国家饲料质量监督检验中心,国家饲料质量监督检验中心,国家饲料质量监督检验中心,国家饲料质量监督检验中心,重庆市兴渝饲料厂,重庆市兴渝饲料厂 北京100081,北京100081,北京100081,北京1
• 出 处: 中国药理学与毒理学杂志
• 发表日期: 2001年05期
• 关键词: 菜籽饼粕 有毒代谢物 饲料产品 饲料蛋白 硫酮 定量检测限 毛细管气相色谱 异硫氰酸酯 腈类 恶唑

本文介绍了高分离度毛细管气相色谱 火焰离子化检测器 (HRCGC FID)监测油菜籽饼粕中 3种主要毒性代谢物异硫氰酸酯 (ITC) ,乙烯基口恶唑烷硫酮(VTO)和腈 (RCN)的改进监测方法 .研究对比了国际标准化组织推荐的ISO 5 5 0 4 1992和其他国家推荐的方法 ,作者改进的HRCGC方法更具广泛的应用范围和较高的灵敏度 ,能测出游离的及酶解后总的毒性代谢物 .定量检测限为 5～ 2 0mg·kg- 1,所以既能测出菜籽饼粕又能测出脱毒后菜籽饼粕及饲料产品中的ITC ,VTO和RCN的含量 ,其结果对于研究饲料蛋白原料菜籽饼粕籽的脱毒工艺的机理极为可贵 .

In this paper, three major toxic metabolites such as isothiocyanate (ITC), vinyl oxazolidine thione (VTO), rapeseed meal and so on were monitored in a rapeseed meal by high resolution capillary gas chromatography with flame ionization detector (HRCGC FID) And nitrile (RCN) .This study compares the ISO 5 5 0 4 1992 recommended by the International Organization for Standardization (ISO) and other countries recommended methods, the improved HRCGC method has more extensive application range and higher sensitivity, can be measured Free and enzymatic total toxic metabolites.Quantitative detection limit of 5 ~ 20mg · kg-1, so both can be measured rapeseed meal and rapeseed meal can be measured after detoxification and feed products ITC, VTO and RCN content, the results for the study of feed protein material rapeseed meal seed detoxification process mechanism is extremely valuable.