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目的研究分析纳米硒对于抗α1-受体抗体介导的糖尿病鼠心肌纤维化的保护作用。方法 70只健康实验室小鼠,选取10只作为对照组,60只小鼠进食24h后,注射50mg/kg链脲佐菌素,连续5d。7d后,采集尾血测定血糖。将40只血糖值>16.65mmol/L的小鼠分为观察组、低剂量组(25μg/kg)、中剂量组(50μg/kg)、高剂量组(100μg/kg),各10只。每天用0.2ml的溶液对各组小鼠进行灌胃,对相应剂量的纳米硒及生理盐水进行补充。2个月后对比小鼠心肌指标。结果观察组、低、中、高剂量组的体重、血糖、MDA、细胞凋亡度以及Bax蛋白表达水平、心肌GSH-PX活性、Bcl-2以及SOD与对照组对比,差异有统计学意义(P<0.05)。结论纳米硒元素能够有效调节血糖,增强体内Bcl-2蛋白表达,其对抗α1-受体抗体介导的糖尿病鼠心肌纤维化的保护作用明显。
Objective To study the protective effect of nano-selenium on myocardial fibrosis induced by anti-α1-receptor antibody in diabetic rats. Methods Totally 70 healthy mice were selected as the control group. Sixty mice were fed with streptozotocin (50mg / kg streptozotocin) for 24 hours after feeding for 5 days. After 7d, the tail blood was collected to measure blood sugar. 40 mice with blood glucose> 16.65mmol / L were divided into observation group, low dose group (25μg / kg), middle dose group (50μg / kg) and high dose group (100μg / kg) The mice in each group were orally administered with 0.2 ml of solution daily, and the corresponding doses of nano-selenium and saline were supplemented. After 2 months, the myocardial indexes of mice were compared. Results The body weight, blood glucose, MDA, apoptosis, Bax protein expression, myocardial GSH-PX activity, Bcl-2 and SOD in the low, middle and high dose groups were significantly different from those in the control group P <0.05). Conclusion Nano-selenium can effectively regulate blood glucose and enhance the expression of Bcl-2 in vivo. It has a protective effect against α1 -receptor antibody-induced myocardial fibrosis in diabetic rats.