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目的探讨电离辐射联合自噬抑制剂或诱导剂对人宫颈癌细胞株增殖的影响。方法采用四甲基偶氮唑蓝(MTT)和流式细胞术(FCM)检测经不同剂量(0、2、4、6、8和10 Gy)照射和6 Gy+自噬抑制剂三甲基腺嘌呤(3-MA)及6Gy+自噬诱导剂雷帕霉素(rapamycin)不同方法处理的人宫颈癌细胞存活和增殖情况,并分析其量效和时效关系。结果随着照射剂量的增加(2、4、6、8和10 Gy)及时间的延长(24、48和72 h),宫颈癌细胞增殖的抑制作用组间比较差异有统计学意义(P<0.05或P<0.01)。6 Gy+rapamycin处理对癌细胞增殖的抑制率低于单纯6Gy照射组(P<0.05),组间比较差异有统计学意义(P<0.05或P<0.01)。6 Gy+3-MA处理对癌细胞增殖的抑制高于单纯6 Gy照射组(P<0.05),处理后24、48和72 h其抑制率组间比较差异有统计学意义(P<0.05或P<0.01)。结论在Hela细胞中电离辐射诱导自噬能够拮抗凋亡,电离辐射联合自噬诱导剂能够抑制Hela细胞凋亡,而电离辐射联合自噬抑制剂能够促进其凋亡。
Objective To investigate the effects of ionizing radiation combined with inhibitors or inducers of autophagy on the proliferation of human cervical cancer cell lines. Methods Tetramethylpyrazolyl blue (MTT) and flow cytometry (FCM) were used to detect the effects of different doses (0, 2, 4, 6, 8 and 10 Gy) Survival and proliferation of human cervical cancer cells treated by different methods of purine (3-MA) and rapamycin (6Gy + autophagy inducer) were analyzed. Results The inhibitory effect on the proliferation of cervical cancer cells was significantly different with the increase of radiation dose (2,4,6,8 and 10 Gy) and time (24,48 and 72 h) (P < 0.05 or P <0.01). The inhibition rate of 6 Gy + rapamycin treatment on cancer cell proliferation was lower than that of 6Gy alone irradiation group (P <0.05). There was significant difference between the two groups (P <0.05 or P <0.01). The inhibition of 6 Gy + 3-MA treatment on cancer cell proliferation was higher than that of 6 Gy irradiation alone (P <0.05), and the inhibition rates at 24, 48 and 72 h after treatment were significantly different (P <0.05 or P <0.01). Conclusion Ionizing radiation induced autophagy in Hela cells can antagonize apoptosis. Ionizing radiation combined with autophagy inducer can inhibit Hela cell apoptosis, and ionizing radiation combined with autophagy inhibitor can promote apoptosis.