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分离、纯化尿毒症中分子物质并研究其生物活性。采用反相高效液相色谱技术将初提的中分子组分进一步纯化，并通过提纯猪肾Ｎａ＋、Ｋ＋－ＡＴＰａｓｅ及体外培养正常人外周血单个核细胞来研究其生物活性。结果表明，尿毒症中分子组分第２～３峰经两步反相高效液相色谱提纯出含肽的中分子化合物。该组分浓度在１．０ｇ／Ｌ以上对Ｎａ＋、Ｋ＋－ＡＴＰａｓｅ有抑制作用；浓度在０．２５ｇ／Ｌ以上对淋巴细胞的增殖有抑制作用。结论：尿毒症患者血清中含有高浓度的含肽中分子物质，其对Ｎａ＋、Ｋ＋－ＡＴＰａｓｅ及淋巴细胞增殖有抑制作用
Isolation and purification of molecular substances in uremia and study its biological activity. The primary molecular components were further purified by reversed-phase high-performance liquid chromatography (RP-HPLC), and their biological activities were studied by purifying porcine kidney Na +, K + -ATPase and culturing PBMCs in vitro. The results showed that the molecular components of uremia in 2 to 3 peak by two-step reversed-phase high performance liquid chromatography to extract the peptide-containing compounds. The concentration of above 1.0g / L of Na +, K + -ATPase inhibited; concentration above 0.25g / L of lymphocyte proliferation inhibited. Conclusion: Serum of patients with uremia contains high concentrations of peptide-containing molecular substances, which inhibit the proliferation of Na +, K + -ATPase and lymphocytes