苹果茎痘病毒新疆库尔勒香梨分离物运动蛋白基因的克隆与序列分析

• 作 者: 赵英 牛建新
• 机 构: 石河子大学农学院园艺系,
• 出 处: 植物病理学报
• 发表日期: 2008年04期
• 关键词: 苹果茎痘病毒 库尔勒香梨 分离物 序列分析 梨脉黄病毒 梨石痘病 苹果茎痘病 香梨 植物病毒 斑驳病

Young leaves and shoot with Apple stem pitting virus(ASPV) detected by RT-PCR(reverse transcription-polymerase chain reaction) from Korla pear were used as materials and total RNA was extracted.Two expected fragments had been obtained by RT-PCR with two specific pairs of primers based on the sequence of NC_003462 published in GenBank.The specific fragments had been recovered and cloned,and the white colonies had been screened out.After enzyme digestion of the plasmids isolated,the positive clone was selected to be sequenced.Compared with NC_003462,three ORFs(open reading frame)-ORF2,ORF3 and ORF4 of ASPV with 673 bp,365 bp,276 bp representing homology of 77%,88.52%,86.33% respectively had been obtained.

Young leaves and shoot with Apple stem pitting virus (ASPV) detected by RT-PCR (reverse transcription-polymerase chain reaction) from Korla pear were used as materials and total RNA was extracted. Two expected fragments had been obtained by RT-PCR with two specific pairs of primers based on the sequence of NC_003462 published in GenBank. The specific fragments had been recovered and cloned, and the white colonies had been screened out. After enzyme digestion of the plasmids isolated, the positive clone was selected to be sequenced. Compared with NC_003462, three ORFs (open reading frame) -ORF2, ORF3 and ORF4 of ASPV with 673 bp, 365 bp, 276 bp representing homology of 77%, 88.52%, 86.33% respectively had been obtained.