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目的探讨蕲艾提取液的抗肝纤维化作用及其机制。方法 24只Wistar大鼠随机分为蕲艾组、丹参组、生理盐水组和空白组,每组6只。其中,蕲艾组用20%的蕲艾提取液灌胃,丹参组用丹参注射液灌胃,3组灌液量均为20ml/kg,每天3次,连续3天,末次给药1h后采血取血清,用细胞培养液DMEM配制成体积分数分别为5%、10%和20%的含药培养液。分别检测各体积分数的蕲艾提取液含药培养液对HSC/T6细胞转化生长因子(TGF-β1)、Smad3、Smad7mRNA和蛋白的影响。结果 5%、10%和20%的蕲艾提取液含药血清培养HSC/T6细胞24h后TGF-β1mRNA表达水平分别为空白组的(0.82±0.03)、(0.72±0.03)和(0.67±0.04)倍,蛋白表达水平分别为(0.66±0.09)、(0.34±0.05)和(0.31±0.07)倍,与生理盐水组相比显著降低(P<0.05);且均下调Smad3mRNA和蛋白水平,同时上调Smad7mRNA和蛋白表达。蕲艾提取液含药血清20%组与丹参组相比无统计学差异。结论蕲艾提取液含药血清抑制肝星状细胞TGF-β1和Smad3的表达,上调Smad7的表达,从而抑制HSC/T6活化、增殖,降低胶原的表达及分泌,达到抗纤维化的目的。
Objective To investigate the anti-hepatic fibrosis and its mechanism of extract of Escherichia coli. Methods Twenty-four Wistar rats were randomly divided into two groups: control group, Salvia miltiorrhiza group, saline group and blank group. Among them, the Ai Ai group with 20% Ai Ai extract gavage Salvia miltiorrhizal group by intragastric administration, the three groups of irrigation volume were 20ml / kg, 3 times a day for 3 days, the last administration after 1h blood The serum was taken and used to prepare the drug-containing culture medium with the volume fraction of 5%, 10% and 20% respectively using cell culture medium DMEM. The effects of different volume fractions of Radix Astragali extracts on HSC / T6 cells’ transforming growth factor (TGF-β1), Smad3, Smad7 mRNA and protein were detected. Results The mRNA expression levels of TGF-β1 in HSC / T6 cells cultured for 5 days, 10% and 20% were respectively (0.82 ± 0.03), (0.72 ± 0.03) and (0.67 ± 0.04 (0.66 ± 0.09), (0.34 ± 0.05) and (0.31 ± 0.07) fold, respectively, which were significantly lower than those in the normal saline group (P <0.05), and both downregulated Smad3 mRNA and protein levels Upregulates Smad7 mRNA and protein expression. Ai Ai extract serum containing 20% group compared with the Salvia group was no significant difference. CONCLUSION: Radix Astragali extract serum inhibits the expression of TGF-β1 and Smad3 in hepatic stellate cells and up-regulates the expression of Smad7, thereby inhibiting the activation and proliferation of HSC / T6, reducing the expression and secretion of collagen, and achieving the purpose of anti-fibrosis.