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目的:分析CD4+ CD25high T细胞(Treg)在膀胱癌患者外周血中的分布,初步探讨其临床意义。方法:采用流式细胞术检测59例膀胱癌患者及20名健康人外周血中Treg的比例,探讨其表型特征并分析Treg比例与临床病理特征的关系。结果:与正常对照〔(2.391±1.198)%〕相比,膀胱癌患者外周血中Treg比例明显增加〔(12.429±6.908)%〕,差异有统计学意义,t=3.76,P<0.01;手术后患者外周血中Treg的比例有所下降,与术前比较差异有统计学意义,P<0.05;Treg水平与各临床病理参数之间差异无统计学意义,P>0.05。表型分析显示,Treg高表达CD45RO、HLA-Ⅰ和Foxp3分子,较高水平表达OX40、GITR、CD152、CD95和CD95L,低表达CD80、CD86和CD154分子,基本不表达CD45 RA和CD69分子,HLA-DR的表达水平在个体间差异较大。结论:膀胱癌患者外周血CD4+CD25 high T细胞水平明显升高,可能与肿瘤免疫功能低下及肿瘤发生发展密切相关。
Objective: To analyze the distribution of CD4 + CD25high T cells (Tregs) in peripheral blood of patients with bladder cancer and to investigate its clinical significance. Methods: Flow cytometry was used to detect the percentage of Treg in peripheral blood of 59 patients with bladder cancer and 20 healthy individuals. The phenotypes of Tregs were analyzed. The relationship between Treg and clinicopathological features was analyzed. Results: Compared with the normal control (2.391 ± 1.198)%, the proportion of Treg in the peripheral blood of patients with bladder cancer was significantly increased 〔(12.429 ± 6.908)%〕, the difference was statistically significant, t = 3.76, P <0.01; The proportion of Treg in the peripheral blood of the patients decreased after operation, which was significantly different from that before operation (P <0.05). There was no significant difference between the Treg levels and the clinicopathological parameters (P> 0.05). Phenotypic analysis showed that Tregs overexpressed CD45RO, HLA-I and Foxp3, and expressed higher levels of OX40, GITR, CD152, CD95 and CD95L, low expression of CD80, CD86 and CD154 molecules, The expression level of -DR varies greatly among individuals. Conclusion: The levels of CD4 + CD25 high T cells in peripheral blood of patients with bladder cancer are significantly increased, which may be closely related to the low immune function and tumor development.