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目的 :研究脑出血血液凝固过程中产生凝血酶的神经毒性作用。方法 :90只大鼠分成 :对照组、脑出血组、脑出血水蛭素干预组、凝血酶组和凝血酶 +水蛭素干预组。术后 48h观察大鼠神经功能缺损评分 (NDS) ,检测血肿周边组织髓过氧化物酶 (MPO)的活性、脑水肿含量及TUNEL阳性细胞数量。结果 :局部注入重组水蛭素显著减轻了脑出血大鼠神经功能缺损 ,降低局部MPO活性、减少脑水分含量及TUNEL阳性细胞数量 ;脑内直接注入凝血酶后局部脑水分含量升高 ,MPO活性升高 ,并可检测到大量TUNEL阳性细胞。结论 :凝血酶与脑出血后的脑水肿、白细胞浸润和神经细胞DNA损伤有关
Objective: To study the neurotoxic effect of thrombin during the process of blood coagulation in cerebral hemorrhage. Methods: Ninety rats were divided into control group, intracerebral hemorrhage group, hirudin intervention group, thrombin group and thrombin + hirudin intervention group. Neurological deficit score (NDS) was observed 48h after operation, and the activity of myeloperoxidase (MPO), the content of brain edema and the number of TUNEL positive cells in the perihematoma were detected. Results: Local administration of recombinant hirudin significantly reduced neurological deficits, decreased local MPO activity, decreased brain water content and TUNEL-positive cells. After intracerebral administration of thrombin, local brain water content increased and MPO activity increased High, and can detect a large number of TUNEL positive cells. Conclusion: Thrombin is related to brain edema, leukocyte infiltration and DNA damage in neurons after intracerebral hemorrhage