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AIM:To evaluate the role of APC mutation in gastriccarcinogenesis and to correlate APC mutation withmicrosatellite instability(MSI)in gastric carcinomas.METHODS:APC mutation was measured with multiplexPCR,denaturing gradient gel electrophoresis and DNAsequencing;and MSI was analyzed by PCR-based methods.RESULTS:Sixty-eight cases of sporadic gastric carcinomawere studied for APC mutation at exon 15 and MSI.APCmutaions were detected in 15(22.1%)gastric cancers.Frequence of APC mutation(33.3 %)in intestinal type ofgastric cancer was significantly higher than that in diffusetype(13.1%,P<0.05).On the contrary,no association wasobserved between APC mutation and tumor size,differentiation,depth of invasion,metastasis or clinical stages.Using five microsatellite markers,MSI in at least one locuswas detected in 17 of 68(25 %)of the tumors analyzed.APC mutations were all detected in MSI-L(only one locus,n=9)or MSS(tumor lacking MSI or stable,n=51),but nomutation was found in MSI-H(≥2 loci,n=-8).CONCLUSION:APC mutation is involved in carcinogenesisof intestinal type of gastric cancer and is independent of MSIphenotype but related to the LOH pathway in gastric cancer.
AIM: To evaluate the role of APC mutation in gastriccarcinogenesis and to correlate APC mutation withmicrosatellite instability (MSI) in gastric carcinomas.METHODS: APC mutation was measured with multiplexPCR, denaturing gradient gel electrophoresis and DNAsequencing; and MSI was analyzed by PCR-based methods .RESULTS: Sixty-eight cases of sporadic gastric carcinomawere studied for APC mutation at exon 15 and MSI.APCmutaions were detected in 15 (22.1%) gastric cancers.Frequence of APC mutation (33.3%) in intestinal type ofgastric cancer was significantly higher than that in diffusetype (13.1%, P <0.05) .On the contrary, no association wasobserved between APC mutation and tumor size, differentiation, depth of invasion, metastasis or clinical stages.Using five microsatellite markers, MSI in at least one locuswas detected in 17 of 68 (25%) of the analyzed tumors. APC mutations were all detected in MSI-L (only one locus, n = 9) or MSS (tumor lacking MSI or stable, n = 51) -H (≥2 loci, n = -8 ). CONCLUSION: APC mutation is involved in carcinogenesis of intestinal type of gastric cancer and is independent of MS Phenotype but related to the LOH pathway in gastric cancer.