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甘氨酸、谷氨酸、缬氨酸和苯丙氨酸与硫酸铜发生灵敏的显色反应,形成蓝色配合物,由于最大吸收波长接近,吸收光谱重叠,用传统的光度法难以进行单一组分的测定。本文通过测定甘氨酸、谷氨酸、缬氨酸和苯丙氨酸模拟混合试样在660nm～750 nm波长范围的吸收光谱,采用偏最小二乘法建立校正模型,对其含量进行预测,结合光度法建立同时测定以上氨基酸含量的新方法,方法应用于牛奶样品中甘氨酸、谷氨酸、缬氨酸和苯丙氨酸含量的同时测定。对模拟混合试样,回收率98.46%～98.79%:RPEs为1.67%～2.34%。
Glycine, glutamic acid, valine and phenylalanine react sensitively with copper sulfate to form a blue complex. Due to the proximity of the maximum absorption wavelength, the absorption spectra overlap, making it difficult to carry out a single component The determination. In this paper, the absorption spectrum of the mixed sample of glycine, glutamic acid, valine and phenylalanine in the wavelength range of 660nm ~ 750nm was determined, the calibration model was established by partial least squares, and the content was predicted. Combined with spectrophotometry A new method for simultaneous determination of the above amino acids was established. The method was applied to the simultaneous determination of glycine, glutamic acid, valine and phenylalanine in milk samples. For the simulated mixed samples, the recoveries ranged from 98.46% to 98.79%: RPEs ranged from 1.67% to 2.34%.