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用带有表达质粒ｐＢＶ２２０（含有γ干扰素基因）的大肠杆菌ＤＨ５α株进行发酵培养，３批中试的菌产量平均为１４．１克／Ｌ，γ干扰素的表达量平均为１．０２×１０９ＩＵ／Ｌ，收集的菌体经高压匀质破菌后收集包涵体，用７ｍｏｌ／Ｌ盐酸胍提取干扰素，此过程可去除７８．４％菌体蛋白，而干扰素活性仅损失１０．４１％，粗制干扰素经复性可使干扰素活性提高４０５％，比活也有明显提高，３批平均为１．７６×１０７ＩＵ／ｍｇ蛋白，复性γ干扰素用三步柱层析法进行纯化，其其得率平均为２８．１３％，其比活分别为３．２２、２．７８和３．０１×１０７ＩＵ／ｍｇ蛋白。３批中试的纯化γ干扰素纯度在ＳＤＳ－ＰＡＧＥ测定均＞９９％，高效液相层析（ＨＰＬＣ）测定＞９８％，其它检定项目也均合乎规程要求。
Fermentation was carried out in E. coli DH5α strain harboring the expression plasmid pBV220 (containing interferon gamma gene). The average yield of bacteria in the three batches was 14.1 g / L, and the average expression of interferon gamma was 1.02 × 109IU / L, the collected cells were homogenized by high pressure homogenization after the collection of inclusion bodies, with 7mol / L guanidine hydrochloride extract of interferon, this process can remove 78.4% of the bacterial protein, and interferon activity loss of only 10.41 %, The renaturation of the crude interferon renders the activity of interferon increased by 405%, the specific activity also significantly improved, the average of three batches was 1.76 × 107IU / mg protein, and the renaturation of interferon gamma by three-step chromatography The average yields were 28.13% and their specific activities were 3.22, 2.78 and 3.01 × 107 IU / mg protein, respectively. The purity of the purified interferon gamma in three batches of trials was> 99% by SDS-PAGE and> 98% by HPLC, respectively. Other test items also met the regulatory requirements.