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OBJECTIVE:To investigate the protective effects of electroacupuncture and LGNHFD on cerebral isch-emia-reperfusion injury,and their effects on the ex-pression of cluster of differentiation 34(CD 34),vas-cular endothelial growth factor(VEGF),and basic fi-broblast growth factor(bFGF)in the peripheral tis-sue of the cerebral ischemic semi-dark zone in rats.The influence and mechanism of expression.METHODS:Healthy male Sprague-Dawley rats were randomly divided into the sham operation group,model group,Traditional Chinese Medicine(TCM)group,electroacupuncture group,and acu-puncture group.The cerebral ischemia model was prepared by middle artery occlusion.The elec-troacupuncture and TCM groups received elec-troacupuncture at the“Neiguan(PC6)”,“Baihui(GV20)”,and“Renzhong(GV26)”acupoints from 4 h after modeling,once daily for 14 d.After modeling,the acupuncture and TCM groups were adminis-tered LGNHFD once daily for 14 d.After treatment,the infarct volume and regional cerebral blood flow(rCBF)were measured in each group,the expres-sion of CD34 in the surrounding ischemic penum-bra was determined using immunohistochemistry,and the expression of angiogenesis-related factors in the ischemic hemisphere was detected by west-ern blot.RESULTS:Compared with the sham operation group,rCBF was significantly decreased(P＜0.01)and cerebral infarction volume was significantly in-creased(P＜0.01)in the model group at four time points(5 min and 3,7,and 14 d after modeling).At three postoperative time points(3,7,and 14 d),the number of CD34+cells in the model group were significantly increased(P＜0.01),and the relative expression levels of VEGF,bFGF,and CD34+in the ischemic hemisphere were significantly increased(P＜0.01,P＜0.05).Compared with the model group,the rCBF of the acupuncture group was sig-nificantly increased at 3,7,and 14 d after interven-tion(P＜0.01),while the rCBF of the electroacu-puncture and TCM groups was significantly in-creased at 7 and 14 d after intervention(P＜0.01).After 3,7,and 14 d of intervention,infarct volume was significantly reduced(P＜0.01),the number of CD34+cells was significantly increased(P＜0.01,P＜0.05),and the relative expression levels of VEGF,bFGF,and CD34 in the ischemic hemisphere were significantly increased(P＜0.01,P＜0.05).Com-pared with the electroacupuncture and TCM groups,the rCBF of the acupuncture group was sig-nificantly increased(P＜0.01),the infarct volume was significantly reduced(P＜0.01),and the rela-tive expression levels of VEGF,bFGF,and CD34 were significantly increased(P＜0.05,P＜0.01)after 3,7,and 14 d of intervention.CONCLUSION:Acupuncture therapy is superior to simple drug and electroacupuncture therapy for re-ducing infarct volume,increasing rCBF and CD34 expression in cells,and promoting capillary regen-eration in rats with cerebral ischemia.This may be related to the increased expression of VEGF,bFGF,and CD34—which are related to cerebrovascular neovascularization—in the ischemic hemispheres of such rats.